Skip to main content

Research Repository

Advanced Search

Application of a solid-phase microextraction-gas chromatography-mass spectrometry/metal oxide sensor system for detection of antibiotic susceptibility in urinary tract infection-causing Escherichia coli – A proof of principle study

Drabińska, Natalia; Hewett, Keith; White, Paul; Avison, Matthew; Persad, Raj; Ratcliffe, Norman; de Lacy Costello, Ben

Authors

Natalia Drabińska

Keith Hewett

Paul White Paul.White@uwe.ac.uk
Professor in Applied Statistics

Matthew Avison

Raj Persad

Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science



Abstract

Purpose: Antibiotic resistance is widespread throughout the world and represents a serious health concern. There is an urgent need for the development of novel tools for rapidly distinguishing antibiotic resistant bacteria from susceptible strains. Previous work has demonstrated that differences in antimicrobial susceptibility can be reflected in differences in the profile of volatile organic compounds (VOCs) produced by dissimilar strains. The aim of this study was to investigate the effect of the presence of cephalosporin antibiotics on the VOC profile of extended spectrum beta-lactamase (ESBL) and non-ESBL producing strains of Escherichia coli. Material and methods: In this study, VOCs from strains of Escherichia coli positive and negative for the most commonly encountered ESBL, CTX-M in the presence of cephalosporin antibiotics were assessed using solid-phase microextraction (SPME) coupled with a combined gas chromatography-mass spectrometry/metal oxide sensor (GC-MS/MOS) system. Results: Our proof-of-concept study allowed for distinguishing CTX-M positive and negative bacteria within 2 ​h after the addition of antibiotics. One MOS signal (RT: 22.6) showed a statistically significant three-way interaction (p ​= ​0.033) in addition to significant two-way interactions for culture and additive (p ​= ​0.046) plus time and additive (p ​= ​0.020). There were also significant effects observed for time (p ​= ​0.009), culture (p ​= ​0.030) and additive (p ​= ​0.028). No effects were observed in the MS data. Conclusions: The results of our study showed the potential of VOC analysis using SPME combined with a GC-MS/MOS system for the early detection of CTX-M-producing, antibiotic-resistant E. coli, responsible for urinary tract infections (UTIs).

Journal Article Type Article
Acceptance Date Sep 3, 2021
Online Publication Date Sep 22, 2021
Publication Date Sep 22, 2021
Deposit Date Jul 14, 2022
Publicly Available Date Sep 23, 2022
Journal Advances in Medical Sciences
Electronic ISSN 1898-4002
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 67
Issue 1
Pages 1-9
DOI https://doi.org/10.1016/j.advms.2021.09.001
Keywords Volatile organic compounds; Gas chromatography-mass spectrometry; Metal-oxide sensor; Antibiotic resistance; Urinary tract infection
Public URL https://uwe-repository.worktribe.com/output/9491409
Publisher URL https://www.sciencedirect.com/science/article/pii/S189611262100047X

Files

Application of a solid-phase microextraction-gas chromatography-mass spectrometry/metal oxide sensor system for detection of antibiotic susceptibility in urinary tract infection-causing Escherichia coli – a proof of principle study (493 Kb)
PDF

Licence
http://creativecommons.org/licenses/by-nc-nd/4.0/

Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/

Copyright Statement
This is the author’s accepted manuscript. The final published version is available here: https://www.sciencedirect.com/science/article/pii/S189611262100047X?via%3Dihub





You might also like



Downloadable Citations