Lysophosphatidic acid-functionalised titanium as a superior surface for supporting human osteoblast (MG63) maturation

Mansell, Jason P.; Brown, Judith; Knapp, Jonathan; Faur, Charl; Blom, Ashley W.

Abstract

Covalent modifications of titanium with small molecules
known to promote human osteoblast maturation are
especially attractive in developing superior biomaterials.
An important step in securing competent bone formation
at implant sites is promoting the formation of mature
osteoblasts, either from committed pre-osteoblasts or
from their mesenchymal progenitors. To this end our
research has focussed on identifying molecules that
enhance human osteoblast formation and maturation and
to develop ways of covalently attaching these molecules to
implant surfaces so that they are more likely to withstand
the rigors of the implantation process whilst still retaining
their bioactivity. Herein we report the novel production of
lipid-functionalised titanium using lysophosphatidic acid
or a related compound, (3S) 1-fluoro-3-hydroxy-4-butyl-
1-phosphonate. Both lipids were especially effective at
co-operating with calcitriol to promote human osteoblast
maturation at these modified Ti surfaces in vitro. The novel
findings presented offer enticing new developments towards
the fabrication of next-generation implant devices with
the potential to significantly enhance the osseointegration
process and with it improvements in future prosthesis
performance and longevity.

• Journal Article Type: Article
• Publication Date: May 1, 2012
• Journal: European Cells and Materials
• Print ISSN: 1473-2262
• Publisher: AO Research Institute Davos
• Peer Reviewed: Peer Reviewed
• Volume: 23
• Pages: 348-361
• Keywords: titanium, surface functionalisation, covalent; attachment, lysophosphatidic acid, receptor agonist, active; vitamin D, osteoblast maturation
• Public URL: https://uwe-repository.worktribe.com/output/947810
• Publisher URL: http://www.ecmjournal.org/journal/papers/vol023/pdf/v023a27.pdf