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Fluorophosphonate-functionalised titanium via a pre-adsorbed alkane phosphonic acid: a novel dual action surface finish for bone regenerative applications

Ayre, Wayne Nishio; Scott, Tom; Hallam, Keith; Blom, Ashley W.; Denyer, Stephen; Bone, Heather K.; Mansell, Jason P.


Wayne Nishio Ayre

Tom Scott

Keith Hallam

Ashley W. Blom

Stephen Denyer

Heather K. Bone

Jason Mansell
Associate Professor in Biomedical Sciences


© 2015, Springer Science+Business Media New York. Abstract: Enhancing vitamin D-induced human osteoblast (hOB) maturation at bone biomaterial surfaces is likely to improve prosthesis integration with resultant reductions in the need for revision arthroplasty consequent to aseptic loosening. Biomaterials that are less appealing to microorganisms implicated in implant failures through infection are also highly desirable. However, finding surfaces that enhance hOB maturation to active vitamin D yet deter bacteria remain elusive. In addressing this, we have sought to bio-functionalise titanium (Ti) with lysophosphatidic acid (LPA) and related, phosphatase-resistant, LPA analogues. The impetus for this follows our discovery that LPA co-operates with active vitamin D3 metabolites to secure hOB maturation in vitro including cells grown upon Ti. LPA has also been found, by others, to inhibit virulence factor production and biofilm formation of the human opportunistic pathogen Pseudomonas aeruginosa. Collectively, selected LPA species might offer potential dual-action surface finishes for contemporary bone biomaterials. In attaching a phosphatase-resistant LPA analogue to Ti we took advantage of the affinity of alkane phosphonic acids for TiO 2 . Herein, we provide evidence for the facile development of a dual-action Ti surface for potential orthopaedic and dental applications. Successful conjugation of an LPA analogue (3S)1-fluoro-3-hydroxy-4-(oleoyloxy)butyl-1-phosphonate (FHBP) to the Ti surface was supported through physiochemical characterisation using x-ray photoelectron spectroscopy and secondary ion mass spectrometry. hOB maturation to active vitamin D3 was enhanced for cells grown on FHBP-Ti whilst these same surfaces exhibited clear antiadherent properties towards a clinical isolate of Staphylococcus aureus. Graphical Abstract: [Figure not available: see fulltext.]


Ayre, W. N., Scott, T., Hallam, K., Blom, A. W., Denyer, S., Bone, H. K., & Mansell, J. P. (2016). Fluorophosphonate-functionalised titanium via a pre-adsorbed alkane phosphonic acid: a novel dual action surface finish for bone regenerative applications. Journal of Materials Science: Materials in Medicine, 27(2),

Journal Article Type Article
Acceptance Date Dec 7, 2015
Online Publication Date Dec 24, 2015
Publication Date Feb 1, 2016
Deposit Date Feb 26, 2016
Publicly Available Date Dec 24, 2016
Journal Journal of Materials Science: Materials in Medicine
Print ISSN 0957-4530
Electronic ISSN 1573-4838
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 27
Issue 2
Article Number 36
Keywords titanium, lysophosphatidic acid, active vitamin D, osteoblasts, differentiation, biomaterial functionalization, staphylococcus aureus, auger electron spectroscopy, bacterial attachment, tryptone soya broth, tryptone soya agar, MG63 osteoblast
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Additional Information Additional Information : The final publication is available at Springer via


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