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Alkaline phosphatase binds tenaciously to titanium; implications for biological surface evaluation following bone implant retrieval

Mansell, Jason; Shiel, Anna; Harwood, Carly; Stephens, Dave

Authors

Jason Mansell Jason.Mansell@uwe.ac.uk
Associate Professor in Biomedical Sciences

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Dr Anna Shiel Anna.Shiel@uwe.ac.uk
Bid Developer - Science and Health

Carly Harwood

Dave Stephens



Abstract

© 2017 Elsevier B.V. Enhancing the performance and longevity of titanium (Ti) implants continues to be a significant developmental theme in contemporary biomaterials design. Our specific focus pertains to the surface functionalisation of Ti using the bioactive lipid, lysophosphatidic acid (LPA) and certain phosphatase-resistant analogues of LPA. Coating survivorship to a plethora of testing regimens is required to align with due regulatory process before novel biomaterials can enter clinical trials. One of the key acceptance criteria is coating retention to the physical stresses experienced during implantation. In assessing coating stability to insertion into porcine bone we found that a subsequent in vitro assessment to confirm coating persistence was masked by abundant alkaline phosphatase (ALP) contamination adsorbed to the metal surface. Herein we report that ALP can bind to Ti in a matter of minutes by simply immersing Ti samples in aqueous solutions of the enzyme. We strongly discourage the in vitro monitoring of osteoblast and stromal cell ALP expression when assessing bioactive coating survivorship following Ti implant retrieval form native bone tissue.

Citation

Stephens, D., Harwood, C., Shiel, A. I., Mansell, J. P., Mansell, J., Shiel, A., …Stephens, D. (2017). Alkaline phosphatase binds tenaciously to titanium; implications for biological surface evaluation following bone implant retrieval. Materials Science and Engineering: C, 76, 472-476. https://doi.org/10.1016/j.msec.2017.03.119

Journal Article Type Article
Acceptance Date Mar 15, 2017
Online Publication Date Mar 16, 2017
Publication Date Jul 1, 2017
Journal Materials Science and Engineering C
Print ISSN 0928-4931
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 76
Pages 472-476
DOI https://doi.org/10.1016/j.msec.2017.03.119
Keywords titanium, functionalisation, bone, osteoblasts, alkaline phosphatase, contamination
Public URL https://uwe-repository.worktribe.com/output/883756
Publisher URL http://dx.doi.org/10.1016/j.msec.2017.03.119

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