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Characterisation of a dual species perfusion biofilm model

Steven, Joshua; Nelson, Shona

Authors

Joshua Steven



Abstract

An estimated 65–80% of human infections are biofilm-related. As the biofilm mode of growth significantly contributes to bacterial tolerance of antimicrobial treatment, this has important ramifications for patient morbidity and associated healthcare costs. Biofilm infections are commonly established by more than one bacterial species which demonstrate synergistic interactions and altered behaviour, presenting yet more challenges to effective treatment. The aim of this study was to characterise a new continuous perfusion model for the culture of dual species biofilms of Staphylococcus aureus and Pseudomonas aeruginosa and to compare the antibiotic sensitivities of cells eluted from the system with biofilm cells.
Biofilms of either S. aureus or P. aeruginosa were established within foam plugs, continually perfused with nutrient broth and challenged with the other species after 24 h. Biofilms were disaggregated after a further 24 h and relative species numbers determined by viable counting. Antibiotic susceptibilities of disaggregated biofilm and eluted cells were assessed by disc diffusion.
P. aeruginosa predominated in dual species biofilms, reaching 2 x 109 cfu/biofilm after 48 h. The S. aureus population was consistently lower, even when established before P. aeruginosa challenge (4.47 x 108 cfu/biofilm at 48 h and 5.5 x 108 cfu/biofilm 24 h post-challenge). Antibiotic susceptibility testing demonstrated no significant difference between eluted and disaggregated P. aeruginosa biofilm cells. S. aureus small colony variants (SCVs) were isolated from challenged biofilms, a likely result of co-culture with P. aeruginosa, and of importance due to their resistant phenotype.

Citation

Steven, J., & Nelson, S. (2018, June). Characterisation of a dual species perfusion biofilm model. Poster presented at Microbiology Society Early Career Microbiologists’ Forum Summer Conference

Presentation Conference Type Poster
Conference Name Microbiology Society Early Career Microbiologists’ Forum Summer Conference
Start Date Jun 14, 2018
End Date Jun 15, 2018
Acceptance Date May 1, 2018
Peer Reviewed Peer Reviewed
Keywords characterisation, dual species, perfusion, biofilm model
Public URL https://uwe-repository.worktribe.com/output/868598
Additional Information Title of Conference or Conference Proceedings : Microbiology Society Early Career Microbiologists’ Forum Summer Conference

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