Pharmacodynamics of ciprofloxacin against Pseudomonas aeruginosa planktonic and biofilm‐derived cells

Marques, Claudia; Nelson, Shona

Abstract

© 2019 The Society for Applied Microbiology The influence of growth phase and state on the survival and recovery of Pseudomonas aeruginosa exposed to ciprofloxacin was investigated using batch culture grown planktonic cells and disaggregated biofilm populations. Biofilms were either nonantibiotic exposed or previously exposed to ciprofloxacin before disaggregation and subsequent challenge with ciprofloxacin. Viable counts showed that late stationary phase cells were tolerant to ciprofloxacin over 24h exposure, while all other populations presented a biphasic killing pattern. In contrast, the metabolic activity of planktonic and biofilm-derived cells remained similar to controls during the initial 6h of ciprofloxacin exposure, despite a significant reduction in viable cell numbers. A similar effect was observed when assessing the postantibiotic effect of 1h ciprofloxacin exposure. Thus, although cell reduction occurred, the metabolic status of the cells remained unchanged. The recovery of disaggregated biofilm cells previously exposed to ciprofloxacin was significantly quicker than naïve biofilm cells, and this latter population's recovery was significantly slower than all planktonic populations. Results from this work have implications for our understanding of biofilm-related infections and their resilience to antimicrobial treatment. Significance and Impact of the Study: Removal of biofilms from surfaces and infection sites via disaggregation and induction of dispersion may reverse their antibiotic tolerant state. However, little is known of the recovery of the cells upon disaggregation from biofilms. Driven by this gap in knowledge we quantified the effect of ciprofloxacin on disaggregated biofilms of Pseudomonas aeruginosa, including those previously exposed to ciprofloxacin. Our results provide further insight into bacterial resilience, regrowth, and antimicrobial efficacy, as reduction in cell viability does not directly correlate with the metabolic activity of bacteria at the time of the exposure to antimicrobials. Thus, despite a perceived reduction in viability, the potential for cell persistence and regrowth remains and recovery is quicker upon subsequent exposure to antimicrobial, supporting the increase in resilience and recurrence of infections.

• Journal Article Type: Article
• Acceptance Date: Feb 6, 2019
• Online Publication Date: Feb 11, 2019
• Publication Date: Apr 1, 2019
• Deposit Date: Mar 8, 2019
• Publicly Available Date: Feb 12, 2020
• Journal: Letters in Applied Microbiology
• Print ISSN: 0266-8254
• Electronic ISSN: 1472-765X
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 68
• Issue: 4
• Pages: 350-359
• DOI: https://doi.org/10.1111/lam.13126
• Keywords: biofilms, antibiotics, pseudomonads, resistance, microbial physiology
• Public URL: https://uwe-repository.worktribe.com/output/847809
• Publisher URL: http://dx.doi.org/10.1111/lam.13126
• Additional Information: Additional Information : This is the peer reviewed version of the following article: [Marques, C. and Nelson, S. (2019) Pharmacodynamics of ciprofloxacin against Pseudomonas aeruginosa planktonic and biofilm‐derived cells. Letters In Applied Microbiology. ISSN 0266-8254], which has been published in final form at http://dx.doi.org/10.1111/lam.13126. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
• Contract Date: Mar 8, 2019