Equations to support redox experimentation

Hancock, John T.; Whiteman, Matthew

Abstract

© Springer Science+Business Media, LLC, part of Springer Nature 2019. Working with redox compounds needs to take into account the oxidation and reduction state of the compound under study. This redox state can be influenced by the media in which the compound is found, but will also be influenced by local environments. For example, this may be dictated perhaps by the locality of amino acids in the three dimensional structure of a protein. Therefore, historically, equations have been developed to enable either the redox poise of the environment to be determined, or the redox state of the compound of interest. If a compound is found in the wrong redox state—perhaps inactive—in a cell this has significant ramifications for its role, for example in cell signaling. Here, the use of such equations is discussed, with examples of the relevance to modern redox biology.

Citation

Hancock, J. T., & Whiteman, M. Equations to support redox experimentation. In J. T. Hancock, & M. E. Conway (Eds.), Redox-Mediated Signal Transduction (183-195). Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9463-2_15

• Online Publication Date: May 31, 2019
• Journal: Methods in Molecular Biology
• Peer Reviewed: Not Peer Reviewed
• Volume: 1990
• Pages: 183-195
• Series Title: Methods in Molecular Biology
• Series Number: 1990
• Book Title: Redox-Mediated Signal Transduction
• ISBN: 97814939-94618
• DOI: https://doi.org/10.1007/978-1-4939-9463-2_15
• Keywords: apoptosis cytochrome, hydrogen peroxide, hydrogen sulfide, Nernst equation, redox couple, redox environment, redox state, thiol modifications
• Public URL: https://uwe-repository.worktribe.com/output/846416
• Publisher URL: http://dx.doi.org/10.1007/978-1-4939-9463-2_15