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Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

Atanassov, Plamen; Bestetti, Massimiliano; Cristiani, Pierangela; Trasatti, Stefano Pm; Guerrini, Edoardo; Babanova, Sofia; Grattieri, Matteo; Santoro, Carlo

Authors

Plamen Atanassov

Massimiliano Bestetti

Pierangela Cristiani

Stefano Pm Trasatti

Edoardo Guerrini

Sofia Babanova

Matteo Grattieri

Carlo Santoro



Abstract

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.

Citation

Atanassov, P., Bestetti, M., Cristiani, P., Trasatti, S. P., Guerrini, E., Babanova, S., …Santoro, C. (2015). Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis. Electroanalysis, 27(2), 327-335. https://doi.org/10.1002/elan.201400543

Journal Article Type Article
Acceptance Date Jan 22, 2015
Publication Date Jan 1, 2015
Deposit Date May 1, 2018
Journal Electroanalysis
Print ISSN 1040-0397
Electronic ISSN 1521-4109
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 27
Issue 2
Pages 327-335
DOI https://doi.org/10.1002/elan.201400543
Keywords biosensor, bilirubin oxidase, oxygen measurement, biofilm, biocathode, MFC
Public URL https://uwe-repository.worktribe.com/output/839329
Publisher URL http://dx.doi.org/10.1002/elan.201400543




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