Enzymatic Oxygen Microsensor Based on Bilirubin Oxidase Applied to Microbial Fuel Cells Analysis

Atanassov, Plamen; Bestetti, Massimiliano; Cristiani, Pierangela; Trasatti, Stefano Pm; Guerrini, Edoardo; Babanova, Sofia; Grattieri, Matteo; Santoro, Carlo

Abstract

© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. A selective oxygen biosensor based on bilirubin oxidase (BOx) was developed. The sensor was used for determining oxygen profiles in a membraneless, single-chamber microbial fuel cell (SCMFC), fed with raw wastewater. The linear response of the sensor was optimized by a diffusion layer of silica gel. A computer-controlled stage was used to obtain accurate and precise measurements. Oxygen concentration in biofilms covering electrodes was measured, showing 3mgL-1 of O2 in the bulk solution, decreasing to 0mgL-1 in the cathodic biofilm. The MFC generated power in the range of 0-0.08mW, associated to the oxygen content.

• Journal Article Type: Article
• Acceptance Date: Jan 22, 2015
• Publication Date: Jan 1, 2015
• Deposit Date: May 1, 2018
• Journal: Electroanalysis
• Print ISSN: 1040-0397
• Electronic ISSN: 1521-4109
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 27
• Issue: 2
• Pages: 327-335
• DOI: https://doi.org/10.1002/elan.201400543
• Keywords: biosensor, bilirubin oxidase, oxygen measurement, biofilm, biocathode, MFC
• Public URL: https://uwe-repository.worktribe.com/output/839329
• Publisher URL: http://dx.doi.org/10.1002/elan.201400543
• Contract Date: May 1, 2018