Wholly printed polypyrrole nanoparticle-based biosensors on flexible substrate

Wallace, Gordon G.; Killard, Anthony J.; Innis, Peter C.; Weng, Bo; Morrin, Aoife; Shepherd, Roderick; Crowley, Karl; Killard, Anthony; Innis, Peter; Wallace, Gordon

Abstract

Printing has been widely used in the sensor industry for its speed, low cost and production scalability. In this work we present a wholly-printed polypyrrole (PPy) based biosensor produced by inkjet printing bioinks composed of dispersions of PPy nanoparticles and enzymes onto screen-printed carbon electrodes. Two enzymes, horseradish peroxidase (HRP) or glucose oxidase (GoD) were incorporated into the PPy nanoparticle dispersions to impart biosensing functionality and selectivity into the conducting polymer ink. Further functionality was also introduced by deposition of a permselective ethyl cellulose (EC) membrane using inkjet printing. Cyclic voltammetry (CV) and chrono-amperometry were used to characterize the response of the PPy biosensors to H2O2 and glucose. Results demonstrated the possibility of PPy based biosensor fabrication using the rapid and low cost technique of inkjet printing. The detection range of H2O2 was found to be 10 μM-10 mM and for glucose was 1-5 mM. © The 2014 Royal Society of Chemistry.

• Journal Article Type: Article
• Publication Date: Feb 21, 2014
• Journal: Journal of Materials Chemistry B
• Print ISSN: 2050-750X
• Electronic ISSN: 2050-7518
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 7
• Pages: 793-799
• DOI: https://doi.org/10.1039/c3tb21378a
• Keywords: polypyrrole, nanoparticles, inkjet, printing, biosensor
• Public URL: https://uwe-repository.worktribe.com/output/827040
• Publisher URL: http://dx.doi.org/10.1039/C3TB21378A