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A biosensor for the determination of high density lipoprotein cholesterol employing combined surfactant-derived selectivity and sensitivity enhancements

Killard, Anthony J.; Ahmadraji, Termeh; Gonzalez-Macia, Laura; Killard, Anthony

Authors

Anthony J. Killard

Termeh Ahmadraji

Laura Gonzalez-Macia laura.gonzalez-macia@uwe.ac.uk

Tony Killard Tony.Killard@uwe.ac.uk
Research Professor in Biomedical Sciences



Abstract

High density lipoprotein cholesterol (HDL-C) is a modifiable risk factor in cardiovascular disease and devices suitable for its determination at the point of care are critical to the future management of hypercholesterolaemia. An electrochemical biosensor for measuring HDL-C was developed. The biosensor was based on a homogeneous assay methodology for selective determination of HDL-C in combination with a printed electrochemical sensor for measuring the reduction of hydrogen peroxide at a silver paste electrode. The polyoxyethylene alkylene tribenzylphenyl ether surfactant (Emulgen B-66) was found to be capable of both the selective dissolution of HDL particles, as well as the enhanced electrocatalytic reduction of hydrogen peroxide. The resulting biosensor was shown to have a linear response to HDL-C from 0.5 to 4 mM (r2=0.998) with an average r.s.d. of 7%. The biosensor was also used to analyse HDL-C in thirteen serum samples and had good agreement with a commercial spectrophotometric precipitation-based assay (r=0.7222; p < 0.058).

Journal Article Type Article
Publication Date Jan 1, 2014
Journal Analytical Methods
Print ISSN 1759-9660
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 6
Pages 3975-3981
APA6 Citation Ahmadraji, T., Gonzalez-Macia, L., & Killard, A. (2014). A biosensor for the determination of high density lipoprotein cholesterol employing combined surfactant-derived selectivity and sensitivity enhancements. Analytical Methods, 6, 3975-3981. https://doi.org/10.1039/C3AY42262C
DOI https://doi.org/10.1039/C3AY42262C
Keywords biosensor, cholesterol, surfactant, HDL-C
Publisher URL http://dx.doi.org/10.1039/C3AY42262C
Additional Information Additional Information : Published online first 7 Mar 2014

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