Dr Emmanuel Adukwu Emmanuel.Adukwu@uwe.ac.uk
Deputy Head of Department
Antimicrobial susceptibility and time-kill analysis of single and combined essential oil blends against clinical and non-clinical isolates of bacteria and fungi
Adukwu, E.C; Edwards-Jones, V
Authors
V Edwards-Jones
Abstract
The continued emergence of antibiotic resistant bacteria and infection outbreaks in healthcare and community settings has created a need for development of new antibiotics and exploration of alternative therapeutics. Essential oils (EOs) have been identified as a potential alternative for use as antimicrobial agents. EOs are complex mixtures containing various chemical compounds with reports showing antimicrobial, antiviral, anti-inflammatory, and antifungal properties. In this study, EOs of geranium, manuka, lavender and lemongrass in single and combined blends were investigated for antimicrobial activity against a range of Gram positive bacteria, Gram negative bacteria and fungi. Methods: The agar incorporation and broth dilution assays were used in determining the MIC and MBC of the EOs. The time-kill assay was performed to compare the microbicidal activity of the single essential oils and a blend of two essential oils during several time intervals. The compositions of the single and combined EOs blends were characterized by GC-MS. The major components in the EOs were: citral (Lemongrass EO), citronellol and Citronellyl formate (Geranium EO), 3, 5-pyridinedicarbonitrile and 1s-cis-calamene (Manuka EO), Linalool and Linalyl acetate (Lavender EO). Results:The tested EOs demonstrated antimicrobial activity against the gram positive bacteria including the MRSA,Listeria monocytogenes and Enterococcus sp, as well as Candida sp. at concentrations between 4% (v/v) for manuka, geranium and lavender EOs. However, all the Gram negative bacteria were inhibited by Lemongrass EO at MIC concentrations 0.06 and 0.5%. Blending of Lemongrass EO with the other three EOs at a 1:1 ratio, caused a total inhibition of both gram positive and gram negative bacteria with MICs ranging between
Citation
Adukwu, E., & Edwards-Jones, V. (2014, May). Antimicrobial susceptibility and time-kill analysis of single and combined essential oil blends against clinical and non-clinical isolates of bacteria and fungi. Poster presented at 114th General Meeting of the American Society for Microbiology Conference
Presentation Conference Type | Poster |
---|---|
Conference Name | 114th General Meeting of the American Society for Microbiology Conference |
Start Date | May 17, 2014 |
End Date | May 20, 2014 |
Acceptance Date | Feb 15, 2014 |
Peer Reviewed | Not Peer Reviewed |
Additional Information | Title of Conference or Conference Proceedings : 114th General Meeting of the American Society for Microbiology Conference |
You might also like
Introducing the JMBE themed issue on science communication
(2018)
Journal Article
Fungal wars: The underlying molecular repertoires of combating mycelia
(2018)
Journal Article
Antimicrobial and sporicidal activity of essential oils and citral against B. subtilis
(2017)
Presentation / Conference
Letter to the Editor of the Journal of Microbiological Methods
(2015)
Journal Article