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Modelling of blood component flexibility using quartz crystal microbalance

Efremov, Vitaly; Lakshmanan, Ramji; Byrne, Barry; Cullen, Sinead; Killard, Anthony

Authors

Vitaly Efremov

Ramji Lakshmanan

Barry Byrne

Sinead Cullen



Abstract

Quartz crystal microbalance (QCM) is a sensitive technique for real-time monitoring of cell adsorption, aggregation and cell-to-surface interaction processes. However, cell adhesion time courses are usually considered as merely qualitative, being presented in terms of QCM resonant frequency shift and/or changes in the dissipation parameter, the precise physical meanings of which are not derived. In the present study, a model of cell adhesion to the QCM sensor surface was proposed. The main output parameter of the model is the rigid mass density, Mr, being related to QCM resonant frequency and dissipation with a simple expression. From this, it can be determined that Mr is the mass density of the layer formed by all directly adhered parts of the cell, being rigidly coupled to the sensor surface. We postulate that the Mr(t) value is proportional to the number of cells adhered by the time t, and that the coefficient of this proportionality is strongly dependent on cell-to-surface interaction forces.

Citation

Efremov, V., Lakshmanan, R., Byrne, B., Cullen, S., & Killard, A. (2014). Modelling of blood component flexibility using quartz crystal microbalance. Journal of Biorheology, 28(1), 45-54

Journal Article Type Article
Publication Date May 1, 2014
Journal Journal of Biorheology
Print ISSN 1867-0466
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 28
Issue 1
Pages 45-54
Keywords QCM, adhesion, cell, absorption, viscoelasticity, platelets
Public URL https://uwe-repository.worktribe.com/output/818068
Publisher URL http://www.biorheology.jp/br.html