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The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria

Jackson, Robert W.; Pickup, Roger W.; Rhodes, Glenn; Bosma, Hester; Studholme, David; Arnold, Dawn L.

Authors

Robert W. Jackson

Roger W. Pickup

Glenn Rhodes

Hester Bosma

David Studholme

Dawn Arnold Dawn.Arnold@uwe.ac.uk
Professor in Molecular Plant Pathology



Abstract

Summary: The rulAB operon of Pseudomonas spp. confers fitness traits on the host and has been suggested to be a hotspot for insertion of mobile elements that carry avirulence genes. Here, for the first time, we show that rulB on plasmid pWW0 is a hotspot for the active site-specific integration of related integron-like elements (ILEs) found in six environmental pseudomonads (strains FH1-FH6). Integration into rulB on pWW0 occurred at position 6488 generating a 3bp direct repeat. ILEs from FH1 and FH5 were 9403bp in length and contained eight open reading frames (ORFs), while the ILE from FH4 was 16233bp in length and contained 16 ORFs. In all three ILEs, the first 5.1kb (containing ORFs 1-4) were structurally conserved and contained three predicted site-specific recombinases/integrases and a tetR homologue. Downstream of these resided ORFs of the 'variable side' with structural and sequence similarity to those encoding survival traits on the fitness enhancing plasmid pGRT1 (ILEFH1 and ILEFH5) and the NR-II virulence region of genomic island PAGI-5 (ILEFH4). Collectively, these ILEs share features with the previously described type III protein secretion system effector ILEs and are considered important to host survival and transfer of fitness enhancing and (a)virulence genes between bacteria. © 2013 Society for Applied Microbiology and John Wiley & Sons Ltd.

Journal Article Type Article
Publication Date Jan 1, 2014
Journal Environmental Microbiology
Print ISSN 1462-2912
Electronic ISSN 1462-2920
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 16
Issue 8
Pages 2374-2388
APA6 Citation Pickup, R. W., Jackson, R. W., Rhodes, G., Bosma, H., Studholme, D., Arnold, D. L., …Pickup, R. (2014). The rulB gene of plasmid pWW0 is a hotspot for the site-specific insertion of integron-like elements found in the chromosomes of environmental Pseudomonas fluorescens group bacteria. Environmental Microbiology, 16(8), 2374-2388. https://doi.org/10.1111/1462-2920.12345
DOI https://doi.org/10.1111/1462-2920.12345
Keywords rulB gene, plasmid pWW0, integron-like elements, chromosomes, bacteria
Publisher URL http://dx.doi.org/10.1111/1462-2920.12345

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