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Description of Brenneria roseae sp. nov. and two subspecies, Brenneria roseae subspecies roseae ssp. nov and Brenneria roseae subspecies americana ssp. nov. isolated from symptomatic oak

Brady, Carrie; Hunter, Gavin; Kirk, Susan; Arnold, Dawn L.; Denman, Sandra

Authors

Gavin Hunter

Susan Kirk

Dawn Arnold Dawn.Arnold@uwe.ac.uk
Professor in Molecular Plant Pathology

Sandra Denman



Abstract

Gram-negative, facultatively anaerobic bacteria were isolated from symptomatic oak tissue in the UK and USA. Partial gyrB sequencing placed ten strains in the genus Brenneria, with B. goodwinii as the closest phylogenetic relative. The strains were investigated further using a polyphasic approach including MLSA (based on partial gyrB, rpoB, infB and atpD gene sequences), 16S rRNA gene sequencing, DNA-DNA relatedness studies and both phenotypic and chemotaxonomic assays. The MLSA and 16S rRNA gene analyses separated the strains into two groups based on origin, suggesting that they belong to Brenneria as two novel species. However, the DNA-DNA relatedness values revealed a closer relationship between the groups and indicated that they should belong to the same species. As the two groups of strains from the UK and USA can be differentiated from each other phenotypically and by ERIC PCR fingerprints, it is proposed to classify them as novel subspecies of a novel Brenneria species. The name Brenneria roseae sp. nov. (FRB 222T=LMG 27714T=NCPPB 4581T) is proposed, with Brenneria roseae subsp. roseae ssp. nov. (FRB 222T=LMG 27714T=NCPPB 4581T) for the strains from the UK and Brenneria roseae subsp. americana ssp. nov. (FRB 223T=LMG 27715T=NCPPB 4582T) for the strains from the USA. © 2014.

Journal Article Type Article
Publication Date Jan 1, 2014
Journal Systematic and Applied Microbiology
Print ISSN 0723-2020
Electronic ISSN 1618-0984
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 37
Issue 6
Pages 396-401
APA6 Citation Arnold, D., Brady, C., Hunter, G., Kirk, S., Arnold, D. L., & Denman, S. (2014). Description of Brenneria roseae sp. nov. and two subspecies, Brenneria roseae subspecies roseae ssp. nov and Brenneria roseae subspecies americana ssp. nov. isolated from symptomatic oak. Systematic and Applied Microbiology, 37(6), 396-401. https://doi.org/10.1016/j.syapm.2014.04.005
DOI https://doi.org/10.1016/j.syapm.2014.04.005
Keywords Brenneria, enterobacteriaceae, acute oak decline, MLSA, taxonomy
Publisher URL http://dx.doi.org/10.1016/j.syapm.2014.04.005
Additional Information Additional Information : Available online before print 22 May 2014.
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