Elevated CO2-Induced Responses in Stomata Require ABA and ABA Signaling
Chater, Caspar; Peng, Kai; Movahedi, Mahsa; Dunn, Jessica A.; Walker, Heather J.; Liang, Yun-Kuan; McLachlan, Deirdre; McLachlan, Deirdre H.; Casson, Stuart; Isner, Jean Charles; Wilson, Ian; Neill, Steven J.; Hedrich, Rainer; Gray, Julie E.; Hetherington, Alistair M.
Jessica A. Dunn
Heather J. Walker
Deirdre H. McLachlan
Jean Charles Isner
Ian Wilson Ian2.Wilson@uwe.ac.uk
Steven Neill Steven.Neill@uwe.ac.uk
Julie E. Gray
Alistair M. Hetherington
© 2015 The Authors. An integral part of global environment change is an increase in the atmospheric concentration of CO2 ([CO2]) . Increased [CO2] reduces leaf stomatal apertures and density of stomata that plays out as reductions in evapotranspiration [2-4]. Surprisingly, given the importance of transpiration to the control of terrestrial water fluxes  and plant nutrient acquisition , we know comparatively little about the molecular components involved in the intracellular signaling pathways by which [CO2] controls stomatal development and function . Here, we report that elevated [CO2]-induced closure and reductions in stomatal density require the generation of reactive oxygen species (ROS), thereby adding a new common element to these signaling pathways. We also show that the PYR/RCAR family of ABA receptors [8, 9] and ABA itself are required in both responses. Using genetic approaches, we show that ABA in guard cells or their precursors is sufficient to mediate the [CO2]-induced stomatal density response. Taken together, our results suggest that stomatal responses to increased [CO2] operate through the intermediacy of ABA. In the case of [CO2]-induced reductions in stomatal aperture, this occurs by accessing the guard cell ABA signaling pathway. In both [CO2]-mediated responses, our data are consistent with a mechanism in which ABA increases the sensitivity of the system to [CO2] but could also be explained by requirement for a CO2-induced increase in ABA biosynthesis specifically in the guard cell lineage. Furthermore, the dependency of stomatal [CO2] signaling on ABA suggests that the ABA pathway is, in evolutionary terms, likely to be ancestral.
|Journal Article Type||Article|
|Publication Date||Oct 19, 2015|
|Publisher||Elsevier (Cell Press)|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Hetherington, A. M., Gray, J. E., Neill, S. J., Wilson, I., Isner, J. C., McLachlan, D. H., …Hetherington, A. (2015). Elevated CO2-Induced Responses in Stomata Require ABA and ABA Signaling. Current Biology, 25(20), 2709-2716. https://doi.org/10.1016/j.cub.2015.09.013|
|Keywords||CO2, stomata, ABA signalling|
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