Jeremy M. Henley
SUMOylation of Syntaxin1A regulates presynaptic endocytosis
Henley, Jeremy M.; Craig, Tim J.; Anderson, Dina; Evans, Ashley J.; Girach, Fatima
Authors
Tim Craig Tim.Craig@uwe.ac.uk
Associate Professor in Biomedical Sciences
Dina Anderson
Ashley J. Evans
Fatima Girach
Abstract
Neurotransmitter release from the presynaptic terminal is under very precise spatial and temporal control. Following neurotransmitter release, synaptic vesicles are recycled by endocytosis and refilled with neurotransmitter. During the exocytosis event leading to release, SNARE proteins provide most of the mechanical force for membrane fusion. Here, we show one of these proteins, Syntaxin1A, is SUMOylated near its C-terminal transmembrane domain in an activity-dependent manner. Preventing SUMOylation of Syntaxin1A reduces its interaction with other SNARE proteins and disrupts the balance of synaptic vesicle endo/exocytosis, resulting in an increase in endocytosis. These results indicate that SUMOylation regulates the emerging role of Syntaxin1A in vesicle endocytosis, which in turn, modulates neurotransmitter release and synaptic function.
Citation
Henley, J. M., Craig, T. J., Anderson, D., Evans, A. J., Girach, F., & Henley, J. (2015). SUMOylation of Syntaxin1A regulates presynaptic endocytosis. Scientific Reports, 5, 17669. https://doi.org/10.1038/srep17669
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 3, 2015 |
Publication Date | Dec 4, 2015 |
Journal | Scientific Reports |
Electronic ISSN | 2045-2322 |
Publisher | Nature Research (part of Springer Nature) |
Peer Reviewed | Peer Reviewed |
Volume | 5 |
Pages | 17669 |
DOI | https://doi.org/10.1038/srep17669 |
Keywords | SUMOylation, exocytosis, endocytosis, Syntaxin1A, presynaptic |
Public URL | https://uwe-repository.worktribe.com/output/802294 |
Publisher URL | http://dx.doi.org/10.1038/srep17669 |
Files
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