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Phosphorylation of Syntaxin‐1a by casein kinase 2α (CK2α) regulates presynaptic vesicle exocytosis from the reserve pool

Shi, Vanilla (Hua); Craig, Tim J.; Bishop, Paul; Nakamura, Yasuko; Rocca, Dan; Wilkinson, Kevin A.; Henley, Jeremy M.

Phosphorylation of Syntaxin‐1a by casein kinase 2α (CK2α) regulates presynaptic vesicle exocytosis from the reserve pool Thumbnail


Authors

Vanilla (Hua) Shi

Tim Craig Tim.Craig@uwe.ac.uk
AHoD in Biological Sciences

Paul Bishop

Yasuko Nakamura

Dan Rocca

Kevin A. Wilkinson

Jeremy M. Henley



Abstract

The t-soluble NSF-attachment protein receptor protein Syntaxin-1a (Stx-1a) is abundantly expressed at pre-synaptic terminals where it plays a critical role in the exocytosis of neurotransmitter-containing synaptic vesicles. Stx-1a is phosphorylated by Casein kinase 2α (CK2α) at Ser14, which has been proposed to regulate the interaction of Stx-1a and Munc-18 to control of synaptic vesicle priming. However, the role of CK2α in synaptic vesicle dynamics remains unclear. Here, we show that CK2α over-expression reduces evoked synaptic vesicle release. Furthermore, shRNA-mediated knockdown of CK2α in primary hippocampal neurons strongly enhanced vesicle exocytosis from the reserve pool, with no effect on the readily releasable pool of primed vesicles. In neurons in which endogenous Stx-1a was knocked down and replaced with a CK2α phosphorylation-deficient mutant, Stx-1a(D17A), vesicle exocytosis was also increased. These results reveal a previously unsuspected role of CK2α phosphorylation in specifically regulating the reserve synaptic vesicle pool, without changing the kinetics of release from the readily releasable pool.

Citation

Shi, V. (., Craig, T. J., Bishop, P., Nakamura, Y., Rocca, D., Wilkinson, K. A., & Henley, J. M. (2021). Phosphorylation of Syntaxin‐1a by casein kinase 2α (CK2α) regulates presynaptic vesicle exocytosis from the reserve pool. Journal of Neurochemistry, 156(5), 614-623. https://doi.org/10.1111/jnc.15161

Journal Article Type Article
Acceptance Date Aug 17, 2020
Online Publication Date Aug 27, 2020
Publication Date Mar 1, 2021
Deposit Date Sep 4, 2020
Publicly Available Date Apr 8, 2021
Journal Journal of Neurochemistry
Print ISSN 0022-3042
Electronic ISSN 1471-4159
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 156
Issue 5
Pages 614-623
DOI https://doi.org/10.1111/jnc.15161
Keywords Biochemistry; Cellular and Molecular Neuroscience; Casein kinase 2α; Phosphorylation Syntaxin‐1; Vesicle exocytosis
Public URL https://uwe-repository.worktribe.com/output/6650867
Publisher URL https://onlinelibrary.wiley.com/doi/full/10.1111/jnc.15161
Additional Information Published: 2020-08-27

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