Resilience and limitations of MFC anodic community when exposed to antibacterial agents

Ieropoulos, Ioannis; Obata, Oluwatosin; Greenman, John; Kurt, Halil; Chandran, Kartik

Abstract

This study evaluates the fate of certain bactericidal agents introduced into microbial fuel cell (MFC) cascades and the response of the microbial community. We tested the response of functioning urine fed MFC cascades using two very different bactericidal agents: a common antibiotic (Ampicillin, 5 g/L) and a disinfectant (Chloroxylenol 4.8 g/L) in concentrations of up to 100 times higher than the usual dose. Results of power generation showed that the established bacteria community was able to withstand high concentrations of ampicillin with good recovery after 24 h of minor decline. However, power generation was adversely affected by the introduction of chloroxylenol, resulting in a 99% loss of power generation. Ampicillin was completely degraded within the MFC cascade (>99.99%), while chloroxylenol remained largely unaffected. Analysis of the microbial community before the addition of the bactericidal agents showed a significant bacterial diversity with at least 35 genera detected within the cascade. Microbial community analysis after ampicillin treatment showed the loss of a small number of bacterial communities and proportional fluctuations of specific strains within the individual MFCs community. On the other hand, there was a significant shift in the bacterial community after chloroxylenol treatment coupled with the loss of at least 13 bacterial genera across the cascade.

• Journal Article Type: Article
• Acceptance Date: Mar 6, 2020
• Online Publication Date: Mar 8, 2020
• Publication Date: Aug 1, 2020
• Deposit Date: Mar 18, 2020
• Publicly Available Date: Mar 9, 2021
• Journal: Bioelectrochemistry
• Print ISSN: 1567-5394
• Electronic ISSN: 1878-562X
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 134
• Article Number: 107500
• DOI: https://doi.org/10.1016/j.bioelechem.2020.107500
• Keywords: Ampicillin; Chloroxylenol; Microbial fuel cell; Anodic biofilm; MFC cascade; Urine
• Public URL: https://uwe-repository.worktribe.com/output/5647119