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Initial trail results of a magnetic biosensor for the rapid detection of Porcine Reproductive and Respiratory Virus (PRRSV) infection

Barnett, Jacqueline M.; Monnier, Bertrand M.; Tyler, Sue; West, David; Ballantine-Dykes, Hugh; Regan, Ed; Wraith, Patrick; Kiely, Janice; Luxton, Richard

Authors

Bertrand Monnier Bertrand.Monnier@uwe.ac.uk
Hourly Paid Instructor HAS DAS - UDAS0001 (grade E)

Sue Tyler

David West

Hugh Ballantine-Dykes

Ed Regan

Patrick Wraith patrickwraith@blueyonder.co.uk

Janice Kiely Janice.Kiely@uwe.ac.uk
Professor in Bio-electronics/Res In CoDi

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor



Abstract

© 2019 The resonant coil magnetometer quantifies paramagnetic particles (PMPs) and has been used to develop magneto-immunoassays in a range of formats. The advantage of magneto-immunoassays is that they are relatively inexpensive, portable, easy to perform and give results in under 5 min. Porcine Reproductive and Respiratory Virus (PRRSV) is an infection of domesticated pigs producing large economic losses in the swine industry current diagnosis is performed using commercially available ELISA kits. Here we describe the development of a competitive magneto-immunoassay (MIA) and pilot study with porcine serum samples. The data show that this technology has the potential for use as a rapid and portable in field system for the detection of antibodies in porcine serum to PRRSV. A range of assay parameters and magnetometer settings were optimised, including the concentration of antibody conjugated PMPs used in the assay and movement of an external magnet to pull particles to a sensor surface. PRRSV positive control serum demonstrated competition with antibody conjugated PMPs with a dose dependent relationship. The magneto-immunoassay developed showed good agreement with the PRRS IDEXX X3 ELISA. The PRRSV magneto-immunoassay demonstrated a sensitivity of 73% and specificity of 100%. The results suggest that a rapid assay using the magnetometer technology detects specific anti-PRRSV antibody in pig serum. The magneto-immunoassay is suitable for use as a rapid ‘on-site’ method for the serological detection of PRRSV infection.

Journal Article Type Article
Publication Date Feb 1, 2020
Journal Sensing and Bio-Sensing Research
Print ISSN 2214-1804
Electronic ISSN 2214-1804
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 27
Pages 100315
APA6 Citation Barnett, J. M., Monnier, B. M., Tyler, S., West, D., Ballantine-Dykes, H., Regan, E., …Luxton, R. (2020). Initial trail results of a magnetic biosensor for the rapid detection of Porcine Reproductive and Respiratory Virus (PRRSV) infection. Sensing and Bio-Sensing Research, 27, 100315. https://doi.org/10.1016/j.sbsr.2019.100315
DOI https://doi.org/10.1016/j.sbsr.2019.100315
Keywords Porcine Reproductive and Respiratory virus Resonant Coil Magnetometer Magneto-immunoassay Competitive immunoassay Serology Pig serum
Publisher URL https://www.sciencedirect.com/science/article/pii/S2214180419300947?via%3Dihub

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Initial trail results of a magnetic biosensor for the rapid detection of Porcine Reproductive and Respiratory Virus (PRRSV) infection (1.1 Mb)
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Licence
http://creativecommons.org/licenses/by-nc-nd/4.0/

Copyright Statement
© 2019 Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license
(http://creativecommons.org/licenses/BY-NC-ND/4.0/).







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