Evaluation of antifungal activities and antihaemolytic effects of cinnamon essential oils from leaf and bark on Candida albicans and Candida auris

Tran, Hoang; Adukwu, Emmanuel

Abstract

Candida infections can be considered as a significant source of patient morbidity and mortality. Candida albicans is the most common pathogen causing Candida infections. Candida auris is a newly described pathogen that is associated with multi-drug resistant candidiasis and candidaemia in humans. The antifungal effects of various essential oils and plant compounds have been demonstrated against human pathogenic fungi. In this study, the effect of Cinnamon leaf and bark essential oil (CEOs) was determined against both C. albicans and C. auris. The disc diffusion (direct and vapour), minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) methods were used to determine antifungal activity of the EOs
against selected strains (C. albicans ATCC 10231, C. albicans ATCC 2091 and C. auris NCPF 8971) whilst the mode of action and haemolysin activity of the CEOs were determined using electron microscopy and light microscopy.Disc diffusion assays showed the inhibitory activities of bark CEO was higher than leaf CEO in both
direct and vapour phase. The MICs and MFCs of bark CEOs for all tested strains was below 0.03%, which was lower than the MICs of the leaf CEO (0.06 – 0.13 % v/v) dependent on the strain and the MFCs at 0.25% v/v. In the morphological interference assays, it was observed that the CEOs damaged the cell membrane and inhibited pseudohyphae formation. The haemolysin production assay showed that CEOs can reduce the haemolytic activity of all tested strains. In conclusion, CEOs showed in vitro antifungal and antihaemolytic potential against C. albicans and C. auris.

Citation

Tran, H., & Adukwu, E. (2019, June). Evaluation of antifungal activities and antihaemolytic effects of cinnamon essential oils from leaf and bark on Candida albicans and Candida auris. Poster presented at Microbiology Society ECM Forum Summer Conference, Dublin, Ireland

• Presentation Conference Type: Poster
• Conference Name: Microbiology Society ECM Forum Summer Conference
• Conference Location: Dublin, Ireland
• Start Date: Jun 20, 2019
• End Date: Jun 21, 2019
• Deposit Date: Oct 15, 2020
• Keywords: Candida spp, essential oils
• Public URL: https://uwe-repository.worktribe.com/output/4749794
• Publisher URL: https://microbiologysociety.org/uploads/assets/uploaded/91f3f7ce-cf7c-4eef-b7b471ff0f326a1c.pdf