Community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients

Marino, Poala J.; Wise, Matt P.; Smith, Ann; Marchesi, Julian R.; Riggio, Marcello P.; Lewis, Michael A.O.; Williams, David W.

Abstract

© 2017 Elsevier Inc. Purpose Mechanically ventilated patients are at risk for developing ventilator-associated pneumonia, and it has been reported that dental plaque provides a reservoir of respiratory pathogens that may aspirate to the lungs and endotracheal tube (ETT) biofilms. For the first time, metataxonomics was used to simultaneously characterize the microbiome of dental plaque, ETTs, and non-directed bronchial lavages (NBLs) in mechanically ventilated patients to determine similarities in respective microbial communities and therefore likely associations. Material and methods Bacterial 16S rRNA gene sequences from 34 samples of dental plaque, NBLs, and ETTs from 12 adult mechanically ventilated patients were analyzed. Results No significant differences in the microbial communities of these samples were evident. Detected bacteria were primarily oral species (e.g., Fusobacterium nucleatum, Streptococcus salivarius, Prevotella melaninogenica) with respiratory pathogens (Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus pneumoniae, and Haemophilus influenzae) also in high abundance. Conclusion The high similarity between the microbiomes of dental plaque, NBLs, and ETTs suggests that the oral cavity is indeed an important site involved in microbial aspiration to the lower airway and ETT. As such, maintenance of good oral hygiene is likely to be highly important in limiting aspiration of bacteria in this vulnerable patient group.

Citation

Marino, P. J., Wise, M. P., Smith, A., Marchesi, J. R., Riggio, M. P., Lewis, M. A., & Williams, D. W. (2017). Community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients. Journal of Critical Care, 39, 149-155. https://doi.org/10.1016/j.jcrc.2017.02.020

• Journal Article Type: Article
• Acceptance Date: May 1, 2017
• Online Publication Date: Feb 16, 2017
• Publication Date: Jun 1, 2017
• Deposit Date: Oct 2, 2019
• Publicly Available Date: Oct 4, 2019
• Journal: Journal of Critical Care
• Print ISSN: 0883-9441
• Electronic ISSN: 1557-8615
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 39
• Pages: 149-155
• DOI: https://doi.org/10.1016/j.jcrc.2017.02.020
• Public URL: https://uwe-repository.worktribe.com/output/3453361
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0883944116308577?via%3Dihub#ac0005
• Related Public URLs: http://orca.cf.ac.uk/98629/
• Additional Information: This article is maintained by: Elsevier; Article Title: Community analysis of dental plaque and endotracheal tube biofilms from mechanically ventilated patients; Journal Title: Journal of Critical Care; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jcrc.2017.02.020; Content Type: article; Copyright: © 2017 Elsevier Inc. All rights reserved.