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A novel natural antimicrobial can reduce the in vitro and in vivo pathogenicity of T6SS positive Campylobacter jejuni and campylobacter coli chicken isolates

Sima, Filip; Stratakos, Alexandros Ch.; Ward, Patrick; Linton, Mark; Kelly, Carmel; Pinkerton, Laurette; Stef, Lavinia; Gundogdu, Ozan; Lazar, Veronica; Corcionivoschi, Nicolae

Authors

Filip Sima

Patrick Ward

Mark Linton

Carmel Kelly

Laurette Pinkerton

Lavinia Stef

Ozan Gundogdu

Veronica Lazar

Nicolae Corcionivoschi



Abstract

© 2018 Sima, Stratakos, Ward, Linton, Kelly, Pinkerton, Stef, Gundogdu, Lazar and Corcionivoschi. Human campylobacteriosis is considered one of the most common foodborne diseases worldwide with poultry identified as the main source of infection accounting for 50-80% of human cases. Highly virulent Campylobacter spp., positive for the Type VI secretion system (T6SS), which have an increased ability to adhere to and invade the host gastrointestinal epithelium are highly prevalent in poultry. Multidrug resistant strains of bacteria are rapidly evolving and therefore, new antimicrobials to supplement animal feed that are able to control Campylobacter species, are in great need. The work presented herein indicates that a novel phenolic antimicrobial, Auranta 3001, is able to reduce the adhesion and invasion of human intestinal epithelial cells (HCT-8) by two T6SS positive chicken isolates, C. jejuni RC039 (p < 0.05) and C. coli RC013 (p < 0.001). Exposure of C. jejuni RC039 and C. coli RC013 to Auranta 3001 downregulated the expression of hcp and cetB genes, known to be important in the functionality of T6SS. Furthermore, the reduced adhesion and invasion is associated with a significant decrease in bacterial motility of both isolates (p < 0.05-p < 0.001) in vitro. Most importantly our in vivo results show that Auranta 3001 is able to reduce cecum colonization levels from log 8 CFU/ml to log 2 CFU/ml for C. jejuni RC039 and from log 7 CFU/ml to log 2 CFU/ml for C. coli RC013. In conclusion, this novel antimicrobial is able to reduce the pathogenic properties of T6SS campylobacters in vitro and also to decrease colonization in vivo.

Journal Article Type Article
Publication Date Sep 7, 2018
Journal Frontiers in Microbiology
Print ISSN 1664-302X
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 9
Issue SEP
APA6 Citation Sima, F., Stratakos, A. C., Ward, P., Linton, M., Kelly, C., Pinkerton, L., …Corcionivoschi, N. (2018). A novel natural antimicrobial can reduce the in vitro and in vivo pathogenicity of T6SS positive Campylobacter jejuni and campylobacter coli chicken isolates. Frontiers in Microbiology, 9(SEP), https://doi.org/10.3389/fmicb.2018.02139
DOI https://doi.org/10.3389/fmicb.2018.02139
Publisher URL http://doi.org/10.3389/fmicb.2018.02139

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