Owen J. Harrison
Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study
Harrison, Owen J.; Monnier, Bertrand; Regan, Ed; West, Dave; Dykes, Hugh Ballantine; Davey, Jeffrey S.; Kiely, Janice; Luxton, Richard
Authors
Bertrand Monnier
Ed Regan
Dave West
Hugh Ballantine Dykes
Jeffrey S. Davey
Janice Kiely Janice.Kiely@uwe.ac.uk
Professor in Bio-electronics/Res In CoDi
Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor
Abstract
Expensive, time-consuming and labour-intensive solvent-extraction and liquid-chromatography methods are the industry's current gold standard for antibiotic residue quantification. A novel immunoassay methodology and system for the rapid detection of clinically relevant levels of tetracycline residues found in food-producing animal tissues is described. Anti-tetracycline antibody-coated paramagnetic particles were used for the specific capture of tetracycline in spiked buffer (with and without a 1% pork muscle tissue suspension) and quantified via an analytically-sensitive in-house magnetometer instrument. Detection of tetracycline between 0.1 μg/mL - 1 μg/mL was achieved, with a readout time (including sample treatment) presented in 20 min. The magneto-immunoassay described provides a rapid, low-cost, de-skilled and analytically-sensitive solution for tetracycline screening at the point-of-sampling, with potential applications for other prevalent antibiotic families used in the international farming and food industry.
Journal Article Type | Article |
---|---|
Acceptance Date | May 22, 2023 |
Online Publication Date | May 25, 2023 |
Publication Date | Aug 1, 2023 |
Deposit Date | Jun 15, 2023 |
Publicly Available Date | Jun 19, 2023 |
Journal | Sensing and Bio-Sensing Research |
Print ISSN | 2214-1804 |
Electronic ISSN | 2214-1804 |
Publisher | Elsevier |
Peer Reviewed | Peer Reviewed |
Volume | 41 |
Article Number | 100566 |
DOI | https://doi.org/10.1016/j.sbsr.2023.100566 |
Keywords | Magneto-immunoassay; Magnetometer; Biosensor; Paramagnetic particles; Tetracycline; Antibiotic detection |
Public URL | https://uwe-repository.worktribe.com/output/10867708 |
Publisher URL | https://www.sciencedirect.com/science/article/pii/S2214180423000181?via%3Dihub |
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Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study
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Licence
http://creativecommons.org/licenses/by-nc-nd/4.0/
Publisher Licence URL
http://creativecommons.org/licenses/by-nc-nd/4.0/
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