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Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study

Harrison, Owen J.; Monnier, Bertrand; Regan, Ed; West, Dave; Dykes, Hugh Ballantine; Davey, Jeffrey S.; Kiely, Janice; Luxton, Richard

Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study Thumbnail


Authors

Owen J. Harrison

Bertrand Monnier

Ed Regan

Dave West

Hugh Ballantine Dykes

Jeffrey S. Davey

Janice Kiely Janice.Kiely@uwe.ac.uk
Professor in Bio-electronics/Res In CoDi

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor



Abstract

Expensive, time-consuming and labour-intensive solvent-extraction and liquid-chromatography methods are the industry's current gold standard for antibiotic residue quantification. A novel immunoassay methodology and system for the rapid detection of clinically relevant levels of tetracycline residues found in food-producing animal tissues is described. Anti-tetracycline antibody-coated paramagnetic particles were used for the specific capture of tetracycline in spiked buffer (with and without a 1% pork muscle tissue suspension) and quantified via an analytically-sensitive in-house magnetometer instrument. Detection of tetracycline between 0.1 μg/mL - 1 μg/mL was achieved, with a readout time (including sample treatment) presented in 20 min. The magneto-immunoassay described provides a rapid, low-cost, de-skilled and analytically-sensitive solution for tetracycline screening at the point-of-sampling, with potential applications for other prevalent antibiotic families used in the international farming and food industry.

Journal Article Type Article
Acceptance Date May 22, 2023
Online Publication Date May 25, 2023
Publication Date Aug 1, 2023
Deposit Date Jun 15, 2023
Publicly Available Date Jun 19, 2023
Journal Sensing and Bio-Sensing Research
Print ISSN 2214-1804
Electronic ISSN 2214-1804
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 41
Article Number 100566
DOI https://doi.org/10.1016/j.sbsr.2023.100566
Keywords Magneto-immunoassay; Magnetometer; Biosensor; Paramagnetic particles; Tetracycline; Antibiotic detection
Public URL https://uwe-repository.worktribe.com/output/10867708
Publisher URL https://www.sciencedirect.com/science/article/pii/S2214180423000181?via%3Dihub

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