The use of coated paramagnetic particles as a physical label in a magneto-immunoassay

Hawkins, P.; Luxton, R.; Richardson, J.

Abstract

An ideal label for use in an immunoassay would require no further chemical or electromagnetic stimulation prior to its detection and would be free from interference from the sample matrix. Micron sized paramagnetic particles are able to perturb magnetic fields. This perturbation can be directly detected using a suitable electronic device and is independent of the sample matrix. In this study coated paramagnetic particles were used as a physical label in a non-competitive solid phase 'sandwich' assay for the detection of human transferrin. The transferrin acted as a 'biological bridge' allowing a dose dependant immobilization of the paramagnetic particles to a polyethylene terephthalate solid phase. Quantitation of the paramagnetic label was achieved using an electronic detection system allowing a linear dose response with a femtomolar detection limit (260 fmol). © 2001 Elsevier Science B.V. All rights reserved.

• Presentation Conference Type: Conference Paper (published)
• Publication Date: Nov 7, 2001
• Journal: Biosensors and Bioelectronics
• Print ISSN: 0956-5663
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 16
• Issue: 9-12
• Pages: 989-993
• DOI: https://doi.org/10.1016/S0956-5663%2801%2900201-9
• Keywords: magneto-immunoassay, paramagnetic particles, transferrin, magnetometer, polyethylene terephthalate, solid phase
• Public URL: https://uwe-repository.worktribe.com/output/1084607
• Publisher URL: http://dx.doi.org/10.1016/S0956-5663(01)00201-9
• Additional Information: Additional Information : The technology described in this paper has received international recognition in that a Spanish company has undertaken a collaborative project to develop an environmental measurement system for bacteria in water, based on magnetic detection of paramagnetic particles and was the basis for funding of two further projects sponsored by DSTL.