Metabolomics and metabolic function analysis of the secretome of articular cartilage and isolated chondrocytes in response to pro-inflammatory cytokines

Fellows, C.; Quasnichka, H.; Chowdhury, N.R.; Budd, E.; Skene, D.J.; Mobasheri, A.

Abstract

Chondrocytes rely primarily on glycolysis to meet their energy requirements, but possess the metabolic flexibility to support cell survival and matrix synthesis during periods of nutrient stress, by enhancing glycolysis with mitochondrial respiration. Accessing this ‘spare respiratory capacity’ requires optimal mitochondrial function, but since impaired mitochondrial function is implicated in osteoarthritis (OA), this mechanism may be deficient or attenuated in joint disease. Metabolic adaptation is evident in early-stage OA, however cartilage from late-stage disease does not seem to have this flexibility. A deeper understanding of these complex metabolic pathways may identify new metabolic markers of disease stage, and support therapeutic strategies for treating OA. Metabolomics has the potential to identify underlying metabolic changes, reveal pathological pathways, novel biomarkers and therapeutic targets. The aim of this study was to identify metabolic processes involved in early stage disease by analysis of metabolites and metabolic function in two inflammatory models of cartilage degradation.

• Presentation Conference Type: Conference Paper (published)
• Conference Name: 2018 OARSI World Congress on Osteoarthritis
• Online Publication Date: Apr 16, 2018
• Publication Date: 2018-04
• Deposit Date: Jan 26, 2023
• Journal: Osteoarthritis and Cartilage
• Print ISSN: 1063-4584
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 26
• Issue: Supplement 1
• Pages: S172
• Series Title: Abstracts from the 2018 OARSI World Congress on Osteoarthritis: Promoting Clinical and Basic Research in Osteoarthritis
• DOI: https://doi.org/10.1016/j.joca.2018.02.373
• Keywords: Orthopedics and Sports Medicine; Biomedical Engineering; Rheumatology
• Public URL: https://uwe-repository.worktribe.com/output/10339129
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S1063458418304734?via%3Dihub