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Use of a bioluminescent Pseudomonas aeruginosa strain within an in vitro microbiological system, as a model of wound infection, to assess the antimicrobial efficacy of wound dressings by monitoring light production

Thorn, Robin; Nelson, Shona; Greenman, John

Authors

Robin Thorn Robin2.Thorn@uwe.ac.uk
Associate Professor Molecular Life Sciences



Abstract

A bioluminescent Pseudomonas aeruginosa was incorporated into an in vitro static diffusion method to determine whether light output could be used as a measure of wound dressing efficacy. A significant linear correlation was observed between viable counts and bioluminescence during exponential growth in planktonic culture (r2 = 0.969). Exponential-phase cells were used to inoculate cellulose discs for integration into an in vitro wound model that incorporated a reservoir of serum. A significant linear correlation was found between bioluminescence (photon counts monitored by a low-light camera) and viable counts in this growth environment (r2 = 0.982). Three antimicrobial wound dressings were applied to the surface of freshly prepared sample discs within the wound model, and the kill kinetics were codetermined by photon and viable counts. Quantifiable kill rates gave the same order of efficacy for the three wound dressings using both types of measurement, and a significant linear correlation was shown between photon and viable counts (r2 = 0.873) within this killing environment. Under all defined conditions, a significant linear correlation between bioluminescence and viable counts was shown but the actual slope of the correlation was different, depending on the physicochemical environment of the cells. Hence, significantly more light per cell (P < 0.0001) was produced when cells in discs were exposed to a killing environment compared to a growing environment. As long as defined conditions are employed, the resulting linear correlation enables the state of the system to be continually monitored without disturbance, allowing more immediate and accurate calculations of kill rates without the need for viable counting. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Citation

Greenman, J., Nelson, S. M., Thorn, R. M., Thorn, R., Nelson, S., & Greenman, J. (2007). Use of a bioluminescent Pseudomonas aeruginosa strain within an in vitro microbiological system, as a model of wound infection, to assess the antimicrobial efficacy of wound dressings by monitoring light production. Antimicrobial Agents and Chemotherapy, 51(9), 3217-3224. https://doi.org/10.1128/AAC.00302-07

Journal Article Type Article
Publication Date Sep 1, 2007
Journal Antimicrobial Agents and Chemotherapy
Print ISSN 0066-4804
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 51
Issue 9
Pages 3217-3224
DOI https://doi.org/10.1128/AAC.00302-07
Keywords bioluminescent pseudomonas aeruginosa strain, vitro microbiological system, model of wound infection, antimicrobial efficacy, wound dressings, light production
Public URL https://uwe-repository.worktribe.com/output/1025268
Publisher URL http://dx.doi.org/10.1128/AAC.00302-07