Antioxidative responses in radish (Raphanus sativus L.) plants stressed by copper and lead in nutrient solution and soil

Teklic, Tihana; Hancock, John T.; Engler, Meri; Paradikovic, Nada; Cesar, Vera; Lepedu�, Hrvoje; �tolfa, Ivna; Be�lo, Drago

Abstract

Radish (Raphanus sativus L.) is commonly grown in urban and suburban areas where the soil may be polluted with heavy metals such as Cu or Pb. In this study, short exposure of radish plantlets to 0.5 mM Cu or Pb in nutrient solution (two days) in growth chamber conditions elicited an antioxidative response, measured in terms of lipid peroxidation, protein and proline accumulation, and peroxidase and catalase activity. Longer exposure to Cu
or Pb when radish was grown outdoors for 50 days in pots filled with field soil with different Cu and Pb content
also resulted in higher lipid peroxidation and proline accumulation, and altered protein content and enzyme activity. The tested parameters of radish antioxidative responses to heavy metal stress differed depending on plant part(leaf or hypocotyl) and stress intensity (heavy metal content in growth medium, exposure duration). The reported
data show that plants grown in soil from sites where this crop could be cultivated do show an oxidative stress
response similar but not identical to that seen under laboratory treatment with heavy metals.

• Journal Article Type: Article
• Publication Date: Jan 1, 2008
• Journal: Acta Biologica Cracoviensia Series Botanica
• Publisher: De Gruyter Open
• Peer Reviewed: Peer Reviewed
• Volume: 50
• Issue: 2
• Pages: 79-86
• Keywords: catalase, copper, hypocotyl, guaiacol peroxidase, heavy metals, lead, leaf, oxidative stress, proline, Raphanus sativus L., radish.
• Public URL: https://uwe-repository.worktribe.com/output/1022402