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Transfer of Erwinia toletana and Erwinia iniecta to a novel genus Winslowiella gen. nov. as Winslowiella toletana comb. nov. and Winslowiella iniecta comb. nov. and description of Winslowiella arboricola sp. nov., isolated from bleeding cankers on broadleaf hosts

Brady, Carrie; Kaur, Sundeep; Crampton, Bridget; Maddock, Daniel; Arnold, Dawn; Denman, Sandra

Transfer of Erwinia toletana and Erwinia iniecta to a novel genus Winslowiella gen. nov. as Winslowiella toletana comb. nov. and Winslowiella iniecta comb. nov. and description of Winslowiella arboricola sp. nov., isolated from bleeding cankers on broadleaf hosts Thumbnail


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Carrie Brady Carrie.Brady@uwe.ac.uk
Senior Lecturer - CHSS - SOAS

Sundeep Kaur

Bridget Crampton

Daniel Maddock

Dawn Arnold

Sandra Denman



Abstract

Following a screening campaign of bleeding cankers of broadleaf hosts in Great Britain, numerous bacterial strains were isolated, identified by 16S rRNA and protein-coding gene sequencing and ultimately classified. During the course of the study, several Gram-negative, facultatively anaerobic strains were isolated from bleeding Platanus x acerifolia (London plane) and Tilia x europaea (common lime) cankers that could not be assigned to an existing species. Partial 16S rRNA gene sequencing placed these strains in the genus Erwinia, as a close phylogenetic relative of Erwinia toletana. In an effort to determine the taxonomic position of the strains, a polyphasic approach was followed including genotypic, genomic, phenotypic, and chemotaxonomic assays. Multilocus sequence analysis based on four protein-coding genes (gyrB, rpoB, infB, and atpD) confirmed the phylogenetic position of the strains as a novel taxon of subgroup 3 of the genus Erwinia, along with E. toletana and E. iniecta, and furthermore, provided support for their reclassification in a novel genus. Whole genome comparisons allowed the delimitation of the novel species and also supported the proposed transfer of subgroup 3 species to a novel genus in the Erwiniaeae. Phenotypically the novel species could be differentiated from E. toletana and E. iniecta, and the novel genus could be differentiated from the closely related genera Erwinia and Mixta. Therefore, we propose (1) the reclassification of E. toletana and E. iniecta in a novel genus, Winslowiella gen. nov., as Winslowiella toletana comb. nov. and Winslowiella iniecta comb. nov., with W. toletana comb. nov. as the type species (type strain A37T = CFBP 6631T = ATCC 700880T = CECT 5263T), and (2) classification of the novel strains as Winslowiella arboricola sp. nov. (type strain BAC 15a-03bT = LMG 32576T = NCPPB 4696T).

Citation

Brady, C., Kaur, S., Crampton, B., Maddock, D., Arnold, D., & Denman, S. (2022). Transfer of Erwinia toletana and Erwinia iniecta to a novel genus Winslowiella gen. nov. as Winslowiella toletana comb. nov. and Winslowiella iniecta comb. nov. and description of Winslowiella arboricola sp. nov., isolated from bleeding cankers on broadleaf hosts. Frontiers in Microbiology, 13, 1063107. https://doi.org/10.3389/fmicb.2022.1063107

Journal Article Type Article
Acceptance Date Oct 31, 2022
Online Publication Date Nov 17, 2022
Publication Date Nov 17, 2022
Deposit Date Nov 2, 2022
Publicly Available Date Mar 29, 2024
Journal Frontiers in Microbiology
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 13
Pages 1063107
DOI https://doi.org/10.3389/fmicb.2022.1063107
Keywords Erwinia toletana, Erwinia iniecta, Erwiniaceae, bleeding canker, Tilia
Public URL https://uwe-repository.worktribe.com/output/10110946

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Transfer of Erwinia toletana and Erwinia iniecta to a novel genus Winslowiella gen. nov. as Winslowiella toletana comb. nov. and Winslowiella iniecta comb. nov. and description of Winslowiella arboricola sp. nov., isolated from bleeding cankers on broadle (781 Kb)
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Copyright Statement
This is the author’s accepted manuscript. The final published version is available here: https://www.frontiersin.org/articles/10.3389/fmicb.2022.1063107/full





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