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Description of three novel species of Scandinavium: Scandinavium hiltneri sp. nov., Scandinavium manionii sp. nov. and Scandinavium tedordense sp. nov., isolated from the oak rhizosphere and bleeding cankers of broadleaf hosts

Maddock, Danniel; Brady, Carrie; Kile, Helene; Denman, Sandra; Arnold, Dawn

Description of three novel species of Scandinavium: Scandinavium hiltneri sp. nov., Scandinavium manionii sp. nov. and Scandinavium tedordense sp. nov., isolated from the oak rhizosphere and bleeding cankers of broadleaf hosts Thumbnail


Authors

Danniel Maddock

Profile image of Carrie Brady

Carrie Brady Carrie.Brady@uwe.ac.uk
Senior Lecturer - CHSS - SOAS

Helene Kile

Sandra Denman

Dawn Arnold



Abstract

While investigating the bacterial populations of environmental samples taken from a mix of healthy and Acute Oak Decline afflicted Quercus robur (pedunculate or English oak) rhizosphere soil samples and swabs of bleeding lesions on Tilia spp. (lime) and Quercus rubra (red oak) trees, several strains belonging to the order Enterobacterales were isolated using selective media and enrichment broth. Seven strains from the Q. robur rhizosphere, three strains from Tilia spp. and one from Q. rubra were investigated, with their taxonomic status determined via a polyphasic taxonomic approach. Initially stains were identified as potential members of the recently described genus Scandinavium, based on the partial sequencing of three housekeeping genes. Further analysis of phenotypic traits, including fatty acid profiles, coupled with 16S rRNA gene and phylogenomic analysis of whole genome sequences were applied to a subset of the strains. Phylogenetic and phylogenomic analysis repeatedly placed the isolates in a monophyletic clade within Scandinavium, with four distinct clusters observed, one of which corresponded to Scandinavium goeteborgense, the type species of the genus. The remaining three clusters could be phenotypically and genotypically differentiated from each other and S. goeteborgense. As such, we describe three novel species of the genus, for which we propose the names Scandinavium hiltneri sp. nov. (type strain H11S7T = LMG 32612T = CCUG 76179T), Scandinavium manionii sp. nov. (type strain H17S15T = LMG 32613T = CCUG 76183T) and Scandinavium tedordense sp. nov. (type strain TWS1aT = LMG 32614T = CCUG 76188T). Additionally, the descriptions of the genus Scandinavium and the type species, S. goeteborgense, are emended.

Journal Article Type Article
Acceptance Date Sep 15, 2022
Online Publication Date Oct 11, 2022
Publication Date Oct 11, 2022
Deposit Date Oct 13, 2022
Publicly Available Date Oct 14, 2022
Journal Frontiers in Microbiology
Electronic ISSN 1664-302X
Publisher Frontiers Media
Peer Reviewed Peer Reviewed
Volume 13
Pages 1011653
DOI https://doi.org/10.3389/fmicb.2022.1011653
Keywords oak, rhizosphere, soil, Scandinavium, Enterobacteriaceae, Tilia, AOD
Public URL https://uwe-repository.worktribe.com/output/10036162
Publisher URL https://www.frontiersin.org/articles/10.3389/fmicb.2022.1011653/full
Additional Information The data presented in the study are deposited in GenBank/EMBL/DDBJ under the accession numbers: OM987267 – OM987269 (16S rRNA); ON221904 – ON221914 (infB); ON221882 – ON221892 (atpD); ON221893 – ON221902 (gyrB); JALIGB000000000 – JALIGG000000000 (whole genome). The raw whole genome sequencing data generated in this study has been deposited in the NCBI Sequence Read Archive (SRA) under the accession numbers SRR21315071 - SRR21315076.

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