On the effect of long-term electrical stimulation on three-dimensional cell cultures: Hen embryo brain spheroids

Uroukov, Ivan S.; Bull, Larry

Abstract

A comprehensive dataset of multielectrode array recordings was collected from three-dimensional hen embryo brain cell cultures, termed spheroids, under long-term electrical stimulation. The aim is to understand the ongoing changes in the spiking activity under electrical stimulation within the lifetime of 14-72DIV of the neuronal networks contained therein. The spiking dynamics were analyzed and behavioral characteristics derived. Some effects on spiking patterns and exhaustion were followed in culture lifetime. With respect to the culture development, two main types of spiking exhaustion were found: one which materializes in the form of a drop in the sporadic (tonic) spiking frequency at the later maturation stages; and another associated with decreasing spiking train appearance throughout an experimental period. © 2008 Uroukov and Bull.

• Journal Article Type: Article
• Publication Date: Jul 8, 2008
• Publicly Available Date: Aug 13, 2020
• Journal: Medical Devices: Evidence and Research
• Print ISSN: 1179-1470
• Publisher: Dove Press
• Peer Reviewed: Peer Reviewed
• Volume: 1
• Pages: 1-12
• DOI: https://doi.org/10.2147/mder.s3245
• Keywords: brain, 3D cell culture, spheroids, long-term electrical stimulation, exhaustion, learning, multielectrode array (MEA), neuronal networks, spike generation
• Public URL: https://uwe-repository.worktribe.com/output/1011001
• Publisher URL: https://doi.org/10.2147/MDER.S3245