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Modelling a dynamic magneto-agglutination bioassay

Hughes, Robert; Fishman, Aaron; Lamb-Riddell, Kathryn; Sleigh Muñoz, Valentina; Champneys, Alan; Kiely, Janice; Luxton, Richard

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Authors

Robert Hughes

Aaron Fishman

Kathryn Lamb-Riddell

Valentina Sleigh Muñoz

Alan Champneys

Janice Kiely Janice.Kiely@uwe.ac.uk
Professor in Bio-electronics/Res In CoDi

Richard Luxton Richard.Luxton@uwe.ac.uk
Research Centre Director-IBST/Professor



Abstract

The process of developing an end-to-end model of a magneto-immunoassay is described, simulating the agglutination effect due to the specific binding of bacteria to paramagnetic particles. After establishing the properties of the dose-specific agglutination through direct imaging, a microfluidic assay was used to demonstrate changes in the magnetophoretic transport dynamics of agglutinated clusters via transient inductive magentometer measurements. End-to-end mathematical modelling is used to establish the physical processes underlying the assay. First, a modified form of Becker-Döring nucleation kinetic equations is used to establish a relationship between analyte dose and average cluster size. Next, Stokes flow equations are used to establish a relationship between cluster size and speed of motion within the fluid chamber. This predicts a cluster-size dynamic profile of concentration of PMPs versus time when the magnetic field is switched between the two actuated magnets. Finally, inductive modelling is carried out to predict the response of the magnetometer circuit in response to the dynamics of magnetic clusters. The predictions of this model are shown to agree well with the results of experiments, and to predict the shape of the dose-response curve.

Citation

Hughes, R., Fishman, A., Lamb-Riddell, K., Sleigh Muñoz, V., Champneys, A., Kiely, J., & Luxton, R. (2023). Modelling a dynamic magneto-agglutination bioassay. Biosensors and Bioelectronics, 222, 114745. https://doi.org/10.1016/j.bios.2022.114745

Journal Article Type Article
Acceptance Date Sep 20, 2022
Online Publication Date Sep 28, 2022
Publication Date Feb 15, 2023
Deposit Date Oct 21, 2022
Publicly Available Date Mar 28, 2024
Journal Biosensors & bioelectronics
Print ISSN 0956-5663
Electronic ISSN 1873-4235
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 222
Pages 114745
DOI https://doi.org/10.1016/j.bios.2022.114745
Keywords Electrochemistry; Biomedical Engineering; General Medicine; Biophysics; Biotechnology
Public URL https://uwe-repository.worktribe.com/output/10085001
Publisher URL https://www.sciencedirect.com/science/article/pii/S0956566322007850?via%3Dihub
Additional Information This article is maintained by: Elsevier; Article Title: Modelling a dynamic magneto-agglutination bioassay; Journal Title: Biosensors and Bioelectronics; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.bios.2022.114745; Content Type: article; Copyright: © 2022 The Authors. Published by Elsevier B.V.

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