Outputs (48)

Understanding and managing peak shape for basic solutes in reversed-phase high performance liquid chromatography (2023)
Journal Article

While other separation mechanisms can challenge the dominance of reversed phase (for example in the separation of native proteins), reversed phase liquid chromatography is the method of choice for the analysis of a wide variety of samples. However, b... Read More about Understanding and managing peak shape for basic solutes in reversed-phase high performance liquid chromatography.

Managing sample introduction problems in hydrophilic interaction liquid chromatography (2023)
Journal Article

Sample injection can cause serious problems in hydrophilic interaction liquid chromatography (HILIC) when the injection solvent has higher elution strength than the mobile phase (mp). It can lead to asymmetric peak shapes and poor efficiency. The pro... Read More about Managing sample introduction problems in hydrophilic interaction liquid chromatography.

Influence of metals in the column or instrument on performance in hydrophilic interaction liquid chromatography (2021)
Journal Article

A method is proposed for measuring the relative contribution of extracolumn and column effects to the detrimental interactions which occur between metal-sensitive solutes and the complete HPLC system. The method involves the substitution of a length... Read More about Influence of metals in the column or instrument on performance in hydrophilic interaction liquid chromatography.

But my peaks are not Gaussian! Part 3: Physicochemical causes of peak tailing (2021)
Journal Article

Although symmetric peaks with Gaussian shapes are predicted by models of the chromatographic process, "perfect peaks" are not observed often outside of textbooks. Several physico-chemical phenomena can lead to asymmetric peak shapes, including analyt... Read More about But my peaks are not Gaussian! Part 3: Physicochemical causes of peak tailing.

Alternative mobile phase additives for the characterization of protein biopharmaceuticals in liquid chromatography – Mass spectrometry (2021)
Journal Article

When analyzing large complex protein biopharmaceuticals, ion-pairing agents imparting low pH are widely used as mobile phase additives to improve the chromatographic performance. However, one of the most effective additives in RPLC and HILIC, trifluo... Read More about Alternative mobile phase additives for the characterization of protein biopharmaceuticals in liquid chromatography – Mass spectrometry.

Evaluation of a linear free energy relationship for the determination of the column void volume in hydrophilic interaction chromatography (2021)
Journal Article

The application of a linear free energy relationship (LFER) to a variety of hydrophilic interaction chromatography columns with different bonded ligands and pore sizes was studied in order to determine their void volume Vm. The method was based on th... Read More about Evaluation of a linear free energy relationship for the determination of the column void volume in hydrophilic interaction chromatography.

Managing the column equilibration time in hydrophilic interaction chromatography (2019)
Journal Article

For a wide variety of hydrophilic interaction chromatography stationary phases, a repeatable partial equilibration was demonstrated in gradient elution after purging with as little as 12 column volumes of mobile phase. Relative standard deviations of... Read More about Managing the column equilibration time in hydrophilic interaction chromatography.

Hydrophilic interaction liquid chromatography: An update (2019)
Journal Article

This article is an update on the technique of hydrophilic interaction liquid chromatography (HILIC) and covers recent ideas on the mechanism of separation, and how it may be manipulated to suit the separation of particular sample types. The advantage... Read More about Hydrophilic interaction liquid chromatography: An update.

Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration (2018)
Journal Article

© 2018 Elsevier B.V. The separation of some zwitterionic, basic and neutral antibiotic and antiretroviral compounds was studied using hydrophilic interaction chromatography (HILIC) on bare silica, bonded amide and urea superficially porous phases. T... Read More about Retention characteristics of some antibiotic and anti-retroviral compounds in hydrophilic interaction chromatography using isocratic elution, and gradient elution with repeatable partial equilibration.

A study of column equilibration time in hydrophilic interaction chromatography (2018)
Journal Article

© 2018 Elsevier B.V. The time taken to achieve full column equilibration for isocratic analysis of acidic, basic, and neutral solutes in hydrophilic interaction liquid chromatography (HILIC) was compared using the mobile phase disturbance method, col... Read More about A study of column equilibration time in hydrophilic interaction chromatography.