Lauren Lett
Investigation of urinary volatile organic compounds as novel diagnostic and surveillance biomarkers of bladder cancer
Lett, Lauren; George, Michael; Slater, Rachael; De Lacy Costello, Ben; Ratcliffe, Norman; Garc�a-Fi�ana, Marta; Lazarowicz, Henry; Probert, Chris
Authors
Michael George
Rachael Slater
Benjamin De Lacy Costello Ben.DeLacyCostello@uwe.ac.uk
Associate Professor in Diagnostics and Bio-Sensing Technology
Norman Ratcliffe Norman.Ratcliffe@uwe.ac.uk
Professor in Materials & Sensors Science
Marta Garc�a-Fi�ana
Henry Lazarowicz
Chris Probert
Abstract
Background: The diagnosis and surveillance of urothelial bladder cancer (UBC) require cystoscopy. There is a need for biomarkers to reduce the frequency of cystoscopy in surveillance; urinary volatile organic compound (VOC) analysis could fulfil this role. This cross-sectional study compared the VOC profiles of patients with and without UBC, to investigate metabolomic signatures as biomarkers. Methods: Urine samples were collected from haematuria clinic patients undergoing diagnostic cystoscopy and UBC patients undergoing surveillance. Urinary headspace sampling utilised solid-phase microextraction and VOC analysis applied gas chromatography-mass spectrometry; the output underwent metabolomic analysis. Results: The median participant age was 70 years, 66.2% were male. Of the haematuria patients, 21 had a new UBC diagnosis, 125 had no cancer. In the surveillance group, 75 had recurrent UBC, 84 were recurrence-free. A distinctive VOC profile was observed in UBC patients compared with controls. Ten VOCs had statistically significant abundances useful to classify patients (false discovery rate range 1.9 × 10−7–2.8 × 10−2). Two prediction models were evaluated using internal validation. An eight-VOC diagnostic biomarker panel achieved AUROC 0.77 (sensitivity 0.71, specificity 0.72). A six-VOC surveillance biomarker panel obtained AUROC 0.80 (sensitivity 0.71 and specificity 0.80). Conclusions: Urinary VOC analysis could aid the diagnosis and surveillance of UBC.
Citation
Lett, L., George, M., Slater, R., De Lacy Costello, B., Ratcliffe, N., García-Fiñana, M., …Probert, C. (2022). Investigation of urinary volatile organic compounds as novel diagnostic and surveillance biomarkers of bladder cancer. British Journal of Cancer, 127(2), 329-336. https://doi.org/10.1038/s41416-022-01785-8
Journal Article Type | Article |
---|---|
Acceptance Date | Mar 8, 2022 |
Online Publication Date | Mar 29, 2022 |
Publication Date | Jul 20, 2022 |
Deposit Date | Jul 28, 2022 |
Publicly Available Date | Jul 29, 2022 |
Journal | British Journal of Cancer |
Print ISSN | 0007-0920 |
Electronic ISSN | 1532-1827 |
Publisher | Springer Nature [academic journals on nature.com] |
Peer Reviewed | Peer Reviewed |
Volume | 127 |
Issue | 2 |
Pages | 329-336 |
DOI | https://doi.org/10.1038/s41416-022-01785-8 |
Keywords | Bladder cancer; Diagnostic markers; Molecular medicine; Predictive markers |
Public URL | https://uwe-repository.worktribe.com/output/9722880 |
Publisher URL | https://www.nature.com/articles/s41416-022-01785-8 |
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Investigation of urinary volatile organic compounds as novel diagnostic and surveillance biomarkers of bladder cancer
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Licence
http://creativecommons.org/licenses/by/4.0/
Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/
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