Effects of four commercially available factor Xa proteins on the fluorogenic anti-factor Xa assay when monitoring unfractionated heparin

Harris, Leanne F.; Castro-L�pez, Vanessa; O'Donnell, James; Murray, Brian; Killard, Anthony

Abstract

Four commercially available factor Xa (FXa) reagents were evaluated in a fluorogenic anti-FXa assay. The four reagents - of which three were of human origin and the fourth was bovine - were compared in terms of the resulting assay dynamic ranges, lag times, coefficient of variation and R 2 values, as well as their sensitivity to unfractionated heparin within the therapeutic range of 0-1.2U/ml. Similar performance of reagents in the fluorogenic anti-FXa assay was observed independent of the source of the reagent or its physical state, which may assist in the standardization of coagulation assays in clinical settings. © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.

• Journal Article Type: Article
• Publication Date: Jan 1, 2012
• Publicly Available Date: Jun 8, 2019
• Journal: Blood Coagulation and Fibrinolysis
• Print ISSN: 0957-5235
• Electronic ISSN: 1473-5733
• Publisher: Lippincott, Williams & Wilkins
• Peer Reviewed: Peer Reviewed
• Volume: 23
• Issue: 1
• Pages: 98-103
• DOI: https://doi.org/10.1097/MBC.0b013e32834ddf4d
• Keywords: FXa, bovine, human, fluorogenic anti-FXa assay, UFH, anticoagulant monitoring
• Public URL: https://uwe-repository.worktribe.com/output/967543
• Publisher URL: http://dx.doi.org/10.1097/MBC.0b013e32834ddf4d