Chronocoulometric determination of urea in human serum using an inkjet printed biosensor

Smyth, Malcolm R.; Suman, Suman; O'Reilly, Emmet; Kelly, Michele; Killard, Anthony; Morrin, Aoife

Abstract

A biosensor for the determination of urea in human serum was fabricated using a combination of inkjet printed polyaniline nanoparticles and inkjet printed urease enzyme deposited sequentially onto screen-printed carbon paste electrodes. Chronocoulometry was used to measure the decomposition of urea via the doping of ammonium at the polyaniline-modified electrode surface at -0.3V vs. Ag/AgCl. Ammonium could be measured in the range from 0.1 to 100mM. Urea could be measured by the sensor in the range of 2-12mM (r2=0.98). The enzyme biosensor was correlated against a spectrophotometric assay for urea in 15 normal human serum samples which yielded a correlation coefficient of 0.85. Bland-Altman plots showed that in the range of 5.8-6.6mM urea, the developed sensor had an average positive experimental bias of 0.12mM (

• Journal Article Type: Article
• Publication Date: Jul 4, 2011
• Deposit Date: May 4, 2011
• Publicly Available Date: Nov 15, 2016
• Journal: Analytica Chimica Acta
• Print ISSN: 0003-2670
• Publisher: Elsevier
• Peer Reviewed: Not Peer Reviewed
• Volume: 697
• Issue: 1-2
• Pages: 98-102
• DOI: https://doi.org/10.1016/j.aca.2011.04.036
• Keywords: biosensor, inkjet, polyaniline nanoparticles, urease, chronocoulometry
• Public URL: https://uwe-repository.worktribe.com/output/961088
• Publisher URL: http://dx.doi.org/10.1016/j.aca.2011.04.036
• Contract Date: Nov 15, 2016