Characterization of the Salmonella bacteriophage vB_SenS-Ent1

Turner, Dann; Hezwani, M; Nelson, Shona; Salisbury, Vyv; Reynolds, Darren M.

Abstract

The bacteriophage vB_SenS-Ent1 (Ent1) is a member of the family Siphoviridae of tailed bacteriophages and infects a broad range of serovars of the enteric pathogen Salmonella enterica. The virion particle is composed of an icosahedral head 64 nm in diameter and a flexible, non-contractile tail of 116 × 8.5 nm possessing terminal fibres. The adsorption rate constant at 37 °C is 6.73 × 10−9 ml min−1. Latent and eclipse periods are 25 and 20 min, respectively, and the burst size is 35 progeny particles per cell after 35 min at 37 °C. Sequencing revealed a circularly permuted, 42 391 bp dsDNA genome containing 58 ORFs organized into four major transcriptional units. Comparisons with the genome sequences of other bacteriophages revealed a high level of nucleotide sequence identity and shared orthologous proteins with the Salmonella phages SETP3, SE2 and KS7 (SS3e) and the Escherichia phages K1G, K1H, K1ind1 and K1ind3.

• Journal Article Type: Article
• Publication Date: Sep 1, 2012
• Journal: Journal of General Virology
• Print ISSN: 0022-1317
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 93
• Issue: 9
• Pages: 2046-2056
• DOI: https://doi.org/10.1099/vir.0.043331-0
• Keywords: Salmonella, bacteriophage, vB_SenS-Ent1
• Public URL: https://uwe-repository.worktribe.com/output/944653
• Publisher URL: http://dx.doi.org/10.1099/vir.0.043331-0