A fully integrated microfluidic device for point of care monitoring of antithrombotics

Killard, A. J.; Harris, L. F.; Rainey, P.; Lindahl, T. L.; Killard, Anthony

Abstract

© 2016 The Royal Society of Chemistry. The simplicity and efficiency of point of care diagnostics have revolutionised patient care. Current methods for measuring hypercoagulability often require trained technicians, large blood volumes, and result in long turnaround times. Standard testing for hypercoagulable disorders is performed in the central laboratory using automated coagulation analysers. However the trend is moving towards the development and implementation of point of care testing, as a result of the ever increasing number of patients on antithrombotic therapy. We present a novel microfluidic device and assay for monitoring the effect of two anticoagulants, unfractionated heparin (UFH) and low molecular weight heparin (LMWH). The assay is based on the anti-Xa assay principle but uses fluorescence detection. Our device is a disposable laminate microfluidic strip, fabricated from the cyclic polyolefin (COP), Zeonor®, which is extremely suitable for application to fluorescent device platforms. We present data on the execution of the anti-Xa assay in this microfluidic format, demonstrating that the assay can be used to measure both UFH and LMWH in human plasma samples from 0 to 1 U mL-1, with a rapid result obtained within 30-60 seconds.

• Journal Article Type: Article
• Acceptance Date: Aug 2, 2016
• Online Publication Date: Aug 22, 2016
• Publication Date: Sep 21, 2016
• Deposit Date: Oct 13, 2016
• Publicly Available Date: Aug 22, 2017
• Journal: Analytical Methods
• Print ISSN: 1759-9660
• Electronic ISSN: 1759-9679
• Publisher: Royal Society of Chemistry
• Peer Reviewed: Peer Reviewed
• Volume: 8
• Issue: 35
• Pages: 6500-6505
• DOI: https://doi.org/10.1039/c6ay01566b
• Keywords: surface modifications, factor xa, diagnostics, heparin, chip, fabrication, platforms, substrate, future, assays
• Public URL: https://uwe-repository.worktribe.com/output/919524
• Publisher URL: http://dx.doi.org/10.1039/C6AY01566B
• Contract Date: Oct 13, 2016