Anodic biofilms as the interphase for electroactive bacterial growth on carbon veil

Artyushkova, Kateryna; Roizman, Dan; Doyle, Lucinda Elizabeth; Mohidin, Abeed Fatima; Atanassov, Plamen; Marsili, Enrico; Santoro, Carlo

Abstract

© 2016 American Vacuum Society. The structure and activity of electrochemically active biofilms (EABs) are usually investigated on flat electrodes. However, real world applications such as wastewater treatment and bioelectrosynthesis require tridimensional electrodes to increase surface area and facilitate EAB attachment. The structure and activity of thick EABs grown on high surface area electrodes are difficult to characterize with electrochemical and microscopy methods. Here, the authors adopt a stacked electrode configuration to simulate the high surface and the tridimensional structure of an electrode for large-scale EAB applications. Each layer of the stacked electrode is independently characterized using confocal laser scanning microscopy (CLSM) and digital image processing. Shewanella oneidensis MR-1 biofilm on stacked carbon veil electrodes is grown under constant oxidative potentials (0, +200, and +400mV versus Ag/AgCl) until a stable current output is obtained. The textural, aerial, and volumetric parameters extracted from CLSM images allow tracking of the evolution of morphological properties within the stacked electrodes. The electrode layers facing the bulk liquid show higher biovolumes compared with the inner layer of the stack. The electrochemical performance of S. oneidensis MR-1 is directly linked to the overall biofilm volume as well as connectivity between cell clusters.

• Journal Article Type: Article
• Acceptance Date: Aug 24, 2016
• Publication Date: Sep 1, 2016
• Deposit Date: Apr 23, 2018
• Publicly Available Date: Apr 25, 2018
• Journal: Biointerphases
• Print ISSN: 1934-8630
• Electronic ISSN: 1559-4106
• Publisher: AIP Publishing
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 3
• DOI: https://doi.org/10.1116/1.4962264
• Keywords: electrochemically active biofilms (EABs), stacked carbon electrodes, confocal laser scanning microscopy (CLSM), digital image processing, shewanella oneidensis MR-1
• Public URL: https://uwe-repository.worktribe.com/output/908187
• Publisher URL: http://dx.doi.org/10.1116/1.4962264
• Additional Information: Additional Information : The final published version can be accessed here: http://dx.doi.org/10.1116/1.4962264.
• Contract Date: Apr 23, 2018