Evidence towards the involvement of nitric oxide in drought tolerance of sugarcane

Silveira, Neidiquele M.; Ribeiro, Rafael V.; Hancock, John T.; Frungillo, Lucas; Siasou, Eleni; Marcos, Fernanda C.C.; Salgado, Ione; Machado, Eduardo C.

Abstract

© 2017 Elsevier Masson SAS Exogenous supply of nitric oxide (NO) increases drought tolerance in sugarcane plants. However, little is known about the role of NO produced by plants under water deficit. The aim of this study was to test the hypothesis that drought-tolerance in sugarcane is associated with NO production and metabolism, with the more drought-tolerant genotype presenting higher NO accumulation in plant tissues. The sugarcane genotypes IACSP95-5000 (drought-tolerant) and IACSP97-7065 (drought-sensitive) were submitted to water deficit by adding polyethylene glycol (PEG-8000) in nutrient solution to reduce the osmotic potential to−0.4MPa. To evaluate short-time responses to water deficit, leaf and root samples were taken after 24h under water deficit. The drought-tolerant genotype presented higher root extracellular NO content, which was accompanied by higher root nitrate reductase (NR) activity as compared to the drought-sensitive genotype under water deficit. In addition, the drought-tolerant genotype had higher leaf intracellular NO content than the drought-sensitive one. IACSP95-5000 exhibited decreases in root S-nitrosoglutathione reductase (GSNOR) activity under water deficit, suggesting that S-nitrosoglutathione (GSNO) is less degraded and that the drought-tolerant genotype has a higher natural reservoir of NO than the drought-sensitive one. Those differences in intracellular and extracellular NO contents and enzymatic activities were associated with higher leaf hydration in the drought-tolerant genotype as compared to the sensitive one under water deficit.

• Journal Article Type: Article
• Acceptance Date: Apr 7, 2017
• Online Publication Date: Apr 8, 2017
• Publication Date: Jun 1, 2017
• Deposit Date: Apr 11, 2017
• Publicly Available Date: Apr 8, 2018
• Journal: Plant Physiology and Biochemistry
• Print ISSN: 0981-9428
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 115
• Pages: 354-359
• DOI: https://doi.org/10.1016/j.plaphy.2017.04.011
• Keywords: drought, super cane, nitric oxide, nitrate reductase, S-nitrosoglutathione reductase, NO metabolism, genotype dependent
• Public URL: https://uwe-repository.worktribe.com/output/885368
• Publisher URL: http://doi.org/10.1016/j.plaphy.2017.04.011
• Additional Information: Additional Information : This was from an international visit funded partly by São Paulo Research Foundation for scholarship (Grants no. 2012/ 19167-0; 2015/ 21546-7).
• Contract Date: Apr 11, 2017