Taxonomy and identification of bacteria associated with acute oak decline

Brady, Carrie; Arnold, Dawn L.; McDonald, James; Denman, Sandra

Abstract

© 2017, The Author(s). Acute oak decline (AOD) is a relatively newly described disorder affecting native oak species in Britain. Symptomatic trees are characterised by stem bleeds from vertical fissures, necrotic lesions in the live tissue beneath and larval galleries of the two spotted oak buprestid (Agrilus biguttatus). Several abiotic and biotic factors can be responsible for tree death, however the tissue necrosis and stem weeping is thought to be caused by a combination of bacterial species. Following investigations of the current episode of AOD which began in 2008, numerous strains belonging to several different bacteria in the family Enterobacteriaceae have been consistently isolated from symptomatic tissue. The majority of these enterobacteria were found to be novel species, subspecies and even genera, which have now been formally classified. The most frequently isolated species from symptomatic oak are Gibbsiella quercinecans, Brenneria goodwinii and Rahnella victoriana. Identification of these bacteria is difficult due to similarities in colony morphology, phenotypic profile and 16S rRNA gene sequences. Current identification relies heavily on gyrB gene amplification and sequencing, which is time consuming and laborious. However, newer techniques based on detection of single nucleotide polymorphisms show greater promise for rapid and reliable identification of the bacteria associated with AOD.

• Journal Article Type: Review
• Acceptance Date: May 22, 2017
• Online Publication Date: Jun 16, 2017
• Publication Date: Jul 1, 2017
• Deposit Date: Jun 13, 2017
• Publicly Available Date: Jul 19, 2017
• Journal: World Journal of Microbiology and Biotechnology
• Print ISSN: 0959-3993
• Electronic ISSN: 1573-0972
• Publisher: Springer (part of Springer Nature)
• Peer Reviewed: Peer Reviewed
• Volume: 33
• Issue: 7
• DOI: https://doi.org/10.1007/s11274-017-2296-4
• Keywords: acute oak decline, Brenneria goodwinii, Gibbsiella quercinecans, taxonomy
• Public URL: https://uwe-repository.worktribe.com/output/884786
• Publisher URL: https://doi.org/10.1007/s11274-017-2296-4
• Additional Information: Additional Information : The final publication is available at Springer via https://doi.org/10.1007/s11274-017-2296-4
• Contract Date: Jun 13, 2017