Effects of Lipid Composition on Bilayer Membranes Quantified by All-Atom Molecular Dynamics

Ding, Wei; Palaiokostas, Michail; Wang, Wen; Orsi, Mario

Abstract

© 2015 American Chemical Society. Biological bilayer membranes typically contain varying amounts of lamellar and nonlamellar lipids. Lamellar lipids, such as dioleoylphosphatidylcholine (DOPC), are defined by their tendency to form the lamellar phase, ubiquitous in biology. Nonlamellar lipids, such as dioleoylphosphatidylethanolamine (DOPE), prefer instead to form nonlamellar phases, which are mostly nonbiological. However, nonlamellar lipids mix with lamellar lipids in biomembrane structures that remain overall lamellar. Importantly, changes in the lamellar vs nonlamellar lipid composition are believed to affect membrane function and modulate membrane proteins. In this work, we employ atomistic molecular dynamics simulations to quantify how a range of bilayer properties are altered by variations in the lamellar vs nonlamellar lipid composition. Specifically, we simulate five DOPC/DOPE bilayers at mixing ratios of 1/0, 3/1, 1/1, 1/3, and 0/1. We examine properties including lipid area and bilayer thickness, as well as the transmembrane profiles of electron density, lateral pressure, electric field, and dipole potential. While the bilayer structure is only marginally altered by lipid composition changes, dramatic effects are observed for the lateral pressure, electric field, and dipole potential profiles. Possible implications for membrane function are discussed.

• Journal Article Type: Article
• Acceptance Date: Nov 11, 2015
• Publication Date: Dec 10, 2015
• Deposit Date: Sep 26, 2016
• Publicly Available Date: Nov 11, 2016
• Journal: Journal of Physical Chemistry B
• Print ISSN: 1520-6106
• Electronic ISSN: 1520-5207
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 119
• Issue: 49
• Pages: 15263-15274
• DOI: https://doi.org/10.1021/acs.jpcb.5b06604
• Keywords: lipid, composition, bilayer, membrane, all-atom, molecular, dynamics
• Public URL: https://uwe-repository.worktribe.com/output/841706
• Publisher URL: http://dx.doi.org/10.1021/acs.jpcb.5b06604
• Contract Date: Sep 26, 2016