Gibbsiella greigii sp. nov., a novel species associated with oak decline in the USA

Brady, Carrie; Hunter, Gavin; Kirk, Susan; Arnold, Dawn L.; Denman, Sandra

Abstract

In 2010, cream-coloured, Gram-negative staining, facultatively anaerobic enterobacteria were isolated from a single black oak tree (Quercus kelloggii) exhibiting decline symptoms in southern California, USA. These 12 isolates were tentatively identified as Gibbsiella quercinecans based on partial gyrB sequencing. Closer examination of the strains using multilocus sequence analysis, based on partial sequences of gyrB, rpoB, infB and atpD genes, and almost complete 16S rRNA gene sequencing suggested that the isolates belong to a novel taxon within the genus Gibbsiella with G. quercinecans as their closest phylogenetic relative. DNA-DNA relatedness studies confirmed that the strains belong to a single taxon in Gibbsiella, which can be differentiated from other members of the genus by several phenotypic traits. Therefore, the name Gibbsiella greigii sp. nov. is proposed for this novel species isolated from symptomatic Q. kelloggii in the USA with FRB 224T (=LMG 27716T=NCPPB 4583T) as the type strain. © 2014 .

• Journal Article Type: Article
• Acceptance Date: Jul 11, 2014
• Online Publication Date: Jul 21, 2014
• Publication Date: Sep 1, 2014
• Deposit Date: Sep 3, 2014
• Journal: Systematic and Applied Microbiology
• Print ISSN: 0723-2020
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 37
• Issue: 6
• Pages: 417-422
• DOI: https://doi.org/10.1016/j.syapm.2014.07.002
• Keywords: Gibbsiella, enterobacteriaceae, acute oak decline, quercus kelloggii, MLSA, taxonomy
• Public URL: https://uwe-repository.worktribe.com/output/812522
• Publisher URL: http://dx.doi.org/10.1016/j.syapm.2014.07.002
• Additional Information: Additional Information : Available online before print 21 July 2014
• Contract Date: Nov 15, 2016