Description of Brenneria roseae sp. nov. and two subspecies, Brenneria roseae subspecies roseae ssp. nov and Brenneria roseae subspecies americana ssp. nov. isolated from symptomatic oak

Brady, Carrie; Hunter, Gavin; Kirk, Susan; Arnold, Dawn L.; Denman, Sandra

Abstract

Gram-negative, facultatively anaerobic bacteria were isolated from symptomatic oak tissue in the UK and USA. Partial gyrB sequencing placed ten strains in the genus Brenneria, with B. goodwinii as the closest phylogenetic relative. The strains were investigated further using a polyphasic approach including MLSA (based on partial gyrB, rpoB, infB and atpD gene sequences), 16S rRNA gene sequencing, DNA-DNA relatedness studies and both phenotypic and chemotaxonomic assays. The MLSA and 16S rRNA gene analyses separated the strains into two groups based on origin, suggesting that they belong to Brenneria as two novel species. However, the DNA-DNA relatedness values revealed a closer relationship between the groups and indicated that they should belong to the same species. As the two groups of strains from the UK and USA can be differentiated from each other phenotypically and by ERIC PCR fingerprints, it is proposed to classify them as novel subspecies of a novel Brenneria species. The name Brenneria roseae sp. nov. (FRB 222T=LMG 27714T=NCPPB 4581T) is proposed, with Brenneria roseae subsp. roseae ssp. nov. (FRB 222T=LMG 27714T=NCPPB 4581T) for the strains from the UK and Brenneria roseae subsp. americana ssp. nov. (FRB 223T=LMG 27715T=NCPPB 4582T) for the strains from the USA. © 2014.

• Journal Article Type: Article
• Acceptance Date: Apr 25, 2014
• Online Publication Date: May 22, 2014
• Publication Date: Sep 1, 2014
• Deposit Date: Sep 3, 2014
• Journal: Systematic and Applied Microbiology
• Print ISSN: 0723-2020
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 37
• Issue: 6
• Pages: 396-401
• DOI: https://doi.org/10.1016/j.syapm.2014.04.005
• Keywords: Brenneria, enterobacteriaceae, acute oak decline, MLSA, taxonomy
• Public URL: https://uwe-repository.worktribe.com/output/812499
• Publisher URL: http://dx.doi.org/10.1016/j.syapm.2014.04.005
• Additional Information: Additional Information : Available online before print 22 May 2014.
• Contract Date: Apr 21, 2016