The effects of glucose supplementation on the development of Acinetobacter baumannii biofilms

Salim, Jisa; Adukwu, Emmanuel

Abstract

The Effect of Glucose on The Development of Acinetobacter baumannii Biofilm

Background: Acinetobacter baumannii, is an opportunistic pathogen causing pneumonia, bacteraemia, wound and urinary tract infections with high mortality rates due to multidrug resistance. A. baumannii biofilm infections associated with medical equipment, pose significant challenges in treatment. Biofilm growth in glucose-rich media triggers extracellular polymeric substances production, crucial for biofilm adhesion. Understanding the role of glucose in aiding biofilm development and pathogenesis of A. baumannii is important. This study aims to explore effects of glucose supplementation on the growth and biofilm development of clinical and wild-type A. baumannii isolates.

Methods: Five A. baumannii isolates were assessed for biofilm formation using crystal violet assay, and antibiotic sensitivity was tested using disc diffusion method. Glucose supplementation assays were conducted, and absorbance readings were taken hourly for 24 and 48 hours to construct growth curves.

Results and Conclusion:

Results indicate glucose as a key factor in A. baumannii biofilm formation, affecting both clinical and wild-type isolates. Multidrug resistant clinical isolates displayed significantly higher optical density levels compared to wild-type isolated when grown in glucose-supplemented media for 48 hours. These findings underscore the significance of environmental stressors like glucose in A. baumannii adhesion, highlighting potential targets for intervention in combating its biofilm-related infections.

• Presentation Conference Type: Poster
• Conference Name: Letters in Applied Microbiology Early Career Research Symposium
• Start Date: May 15, 2024
• Deposit Date: Jun 24, 2024
• Publicly Available Date: Jun 26, 2024
• Public URL: https://uwe-repository.worktribe.com/output/12082206