Brenneria goodwinii sp. nov., associated with acute oak decline in the UK

Denman, Sandra; Brady, Carrie; Kirk, Susan; Cleenwerck, Ilse; Venter, Stephanus; Coutinho, Teresa; De Vos, Paul

Abstract

A group of nine Gram-negative staining, facultatively anaerobic bacterial strains isolated from native oak trees displaying symptoms of acute oak decline (AOD) in the UK were investigated using a polyphasic approach. 16S rRNA gene sequencing and phylogenetic analysis revealed that these isolates form a distinct lineage within the genus Brenneria, family Enterobacteriaceae, and are most closely related to Brenneria rubrifaciens (97.6% sequence similarity to the type strain). Multilocus sequence analysis based on four housekeeping genes (gyrB, rpoB, infB and atpD) confirmed their position within the genus Brenneria, while DNA-DNA hybridization indicated that the isolates belong to a single taxon. The isolates can be differentiated phenotypically from their closest phylogenetic neighbours. The phylogenetic and phenotypic data demonstrate that these isolates from oak with symptoms of AOD represent a novel species in the genus Brenneria, for which the name Brenneria goodwinii sp. nov. (type strain FRB 141T 5R-43656T =BCC 845T =LMG 26270T =NCPPB 4484T) is proposed. © G 2012 IUMS.

• Journal Article Type: Article
• Publication Date: Oct 1, 2012
• Deposit Date: Mar 22, 2024
• Publicly Available Date: Mar 27, 2024
• Journal: International Journal of Systematic and Evolutionary Microbiology
• Print ISSN: 1466-5026
• Electronic ISSN: 1466-5034
• Publisher: Microbiology Society
• Peer Reviewed: Peer Reviewed
• Volume: 62
• Issue: 10
• Pages: 2451-2456
• DOI: https://doi.org/10.1099/ijs.0.037879-0
• Public URL: https://uwe-repository.worktribe.com/output/11834194