Development and Evaluation of a Laboratory‐Based Technique for Screening Somaclonal Regenerants of Watercress (Rorippanasturtium‐aquatium) for Resistance to Crook Root Disease (Spongospora subterranea f. sp. nasturtii)

Arnold, D. L.; Arnold, Dawn L.; Flegmann, A. W.; Clarkson, J. M.

Abstract

A laboratory based method has been developed for infecting watercress with the crook root fungus (Spongospora subterranea f.sp. nasturtii). This utilised plastic trays of nutrient solution on which watercress shoots, supported by floats, were grown for a pre‐inoculation period of 14 days before an inoculum consisting of 1 g of crook roots was added. Seven watercress someclones derived from plantlets regenerated from callus of a parental commercial control line (clone A) were tested onthree separate occasions. No variation within the parental control plants was seen but significant variation both between and within the individual somaclones over successive tests was observed. Two somaclones showed increased resistance to crook root disease compared with parental control plants. Copyright © 1994, Wiley Blackwell. All rights reserved

• Journal Article Type: Article
• Publication Date: Jan 1, 1994
• Journal: Journal of Phytopathology
• Print ISSN: 0931-1785
• Electronic ISSN: 1439-0434
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 141
• Issue: 2
• Pages: 202-208
• DOI: https://doi.org/10.1111/j.1439-0434.1994.tb01462.x
• Public URL: https://uwe-repository.worktribe.com/output/1108620
• Publisher URL: http://dx.doi.org/10.1111/j.1439-0434.1994.tb01462.x