Rapid detection of tetracycline residues at clinically relevant levels via a novel, inexpensive and analytically-sensitive magneto-immunoassay – A feasibility study

Harrison, Owen J.; Monnier, Bertrand; Regan, Ed; West, Dave; Dykes, Hugh Ballantine; Davey, Jeffrey S.; Kiely, Janice; Luxton, Richard

Abstract

Expensive, time-consuming and labour-intensive solvent-extraction and liquid-chromatography methods are the industry's current gold standard for antibiotic residue quantification. A novel immunoassay methodology and system for the rapid detection of clinically relevant levels of tetracycline residues found in food-producing animal tissues is described. Anti-tetracycline antibody-coated paramagnetic particles were used for the specific capture of tetracycline in spiked buffer (with and without a 1% pork muscle tissue suspension) and quantified via an analytically-sensitive in-house magnetometer instrument. Detection of tetracycline between 0.1 μg/mL - 1 μg/mL was achieved, with a readout time (including sample treatment) presented in 20 min. The magneto-immunoassay described provides a rapid, low-cost, de-skilled and analytically-sensitive solution for tetracycline screening at the point-of-sampling, with potential applications for other prevalent antibiotic families used in the international farming and food industry.

• Journal Article Type: Article
• Acceptance Date: May 22, 2023
• Online Publication Date: May 25, 2023
• Publication Date: Aug 1, 2023
• Deposit Date: Jun 15, 2023
• Publicly Available Date: Jun 19, 2023
• Journal: Sensing and Bio-Sensing Research
• Print ISSN: 2214-1804
• Electronic ISSN: 2214-1804
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 41
• Article Number: 100566
• DOI: https://doi.org/10.1016/j.sbsr.2023.100566
• Keywords: Magneto-immunoassay; Magnetometer; Biosensor; Paramagnetic particles; Tetracycline; Antibiotic detection
• Public URL: https://uwe-repository.worktribe.com/output/10867708
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S2214180423000181?via%3Dihub