Molecular evaluation of the metabolism of estrogenic di(2-ethylhexyl) phthalate in Mycolicibacterium sp.

Bhattacharyya, Mousumi; Dhar, Rinita; Basu, Suman; Das, Avijit; Reynolds, Darren M.; Dutta, Tapan K.

Abstract

Background: Di(2-ethylhexyl) phthalate (DEHP) is a widely detected plasticizer and a priority pollutant of utmost concern for its adverse impact on humans, wildlife and the environment. To eliminate such toxic burden, biological processes are the most promising ways to combat rampant environmental insults under eco-friendly conditions. The present study investigated the biochemical and molecular assessment of the catabolic potential of Mycolicibacterium sp. strain MBM in the assimilation of estrogenic DEHP. Results: A detailed biochemical study revealed an initial hydrolytic pathway of degradation for DEHP followed by the assimilation of hydrolyzed phthalic acid and 2-ethylhexanol to TCA cycle intermediates. Besides the inducible nature of DEHP-catabolic enzymes, strain MBM can efficiently utilize various low- and high-molecular-weight phthalate diesters and can grow under moderately halotolerant conditions. Whole genome sequence analysis exhibited a genome size of 6.2Mb with a GC content of 66.51% containing 6,878 coding sequences, including multiple genes, annotated as relevant to the catabolism of phthalic acid esters (PAEs). Substantiating the annotated genes through transcriptome assessment followed by RT-qPCR analysis, the possible roles of upregulated genes/gene clusters in the metabolism of DEHP were revealed, reinforcing the biochemical pathway of degradation at the molecular level. Conclusions: A detailed co-relation of biochemical, genomic, transcriptomic and RT-qPCR analyses highlights the PAE-degrading catabolic machineries in strain MBM. Further, due to functional attributes in the salinity range of both freshwater and seawater, strain MBM may find use as a suitable candidate in the bioremediation of PAEs.

• Journal Article Type: Article
• Acceptance Date: Apr 12, 2023
• Online Publication Date: Apr 27, 2023
• Publication Date: Dec 1, 2023
• Deposit Date: May 18, 2023
• Publicly Available Date: May 18, 2023
• Journal: Microbial Cell Factories
• Electronic ISSN: 1475-2859
• Publisher: BioMed Central
• Peer Reviewed: Peer Reviewed
• Volume: 22
• Issue: 1
• Pages: 82
• DOI: https://doi.org/10.1186/s12934-023-02096-0
• Keywords: Research, Plasticizer, Phthalates, Biodegradation, Mycolicibacterium, Estrogenic chemicals, Genome, Transcriptome
• Public URL: https://uwe-repository.worktribe.com/output/10723736
• Publisher URL: https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-023-02096-0