A comparative study of the analysis of human urine headspace using gas chromatography-mass spectrometry

Smith, Stephen; Burden, H.; de Lacy Costello, Ben; Persad, R.; Ratcliffe, Norman M.; Whittington, K.; Probert, C. S.

Abstract

First-void urine samples were obtained from 24 elderly, asymptomatic men (median age 62.9 years). The headspace above pH adjusted urine samples were extracted using a carboxen/polydimethylsiloxane solid phase micro-extraction fibre and the volatile organic compounds analysed by gas chromatography/mass spectrometry. A total of 147 compounds were identified in the headspace of urine. The acidified samples recorded a total of 92 compounds, 27 of which were ubiquitous, basified samples 70 compounds, with 12 ubiquitous and unmodified pH samples 49, with 6 ubiquitous. Five compounds were ubiquitous irrespective of pH: acetone, methylene chloride, 4-heptanone, 2-pentanone and 2-butanone. A comparative analysis of unfrozen and frozen-thawed urine (stored at room temperature for 0, 1 and 8 h) showed that samples retained the same number of compounds although variations in the peak areas for some compounds were observed. © 2008 IOP Publishing Ltd.

• Journal Article Type: Article
• Publication Date: Sep 1, 2008
• Journal: Journal of Breath Research
• Print ISSN: 1752-7155
• Electronic ISSN: 1752-7163
• Publisher: IOP Publishing
• Peer Reviewed: Peer Reviewed
• Volume: 2
• Issue: 3
• Pages: 037022
• DOI: https://doi.org/10.1088/1752-7155/2/3/037022
• Public URL: https://uwe-repository.worktribe.com/output/1022081
• Publisher URL: http://dx.doi.org/10.1088/1752-7155/2/3/037022