Dr Tim Craig's Outputs (33)

SUMOylation is required for glycine-induced increases in AMPA receptor surface expression (ChemLTP) in hippocampal neurons (2013)
Journal Article

Multiple pathways participate in the AMPA receptor trafficking that underlies long-term potentiation (LTP) of synaptic transmission. Here we demonstrate that protein SUMOylation is required for insertion of the GluA1 AMPAR subunit following transient... Read More about SUMOylation is required for glycine-induced increases in AMPA receptor surface expression (ChemLTP) in hippocampal neurons.

SUMOylation, Arc and the regulation homeostatic synaptic scaling: Implications in health and disease (2012)
Journal Article

Neurons compensate for changes in network activity by altering the sensitivity of transmission across collections of synapses by up- or downregulating the number of synaptic AMPA receptors. We recently reported that, in parallel to increasing AMPA re... Read More about SUMOylation, Arc and the regulation homeostatic synaptic scaling: Implications in health and disease.

Homeostatic Synaptic Scaling Is Regulated by Protein SUMOylation (2012)
Journal Article

Background: SUMOylation regulates many cell pathways.
Results: Synaptic scaling elicited by suppression of neuronal activity decreases the deSUMOylating enzyme SENP1; overexpression of SENP1 prevents synaptic scaling.
Conclusion: SUMOylation is req... Read More about Homeostatic Synaptic Scaling Is Regulated by Protein SUMOylation.

Protein SUMOylation in spine structure and function (2011)
Journal Article

The active regulation of spine structure and function is of fundamental importance for information storage in the brain. Many proteins involved in spine development and activity-dependent remodelling are potential or validated substrates for modifica... Read More about Protein SUMOylation in spine structure and function.

A proposal of combined evaluation of waist circumference and BMI for the diagnosis of metabolic syndrome (2009)
Journal Article

We performed a receiver operator characteristic (ROC) curve analysis of 3915 men and 2032 women. Subjects who were diagnosed with two or more factors among high blood pressure, hyperglycaemia or high triglyceride and/or low HDL were classified as the... Read More about A proposal of combined evaluation of waist circumference and BMI for the diagnosis of metabolic syndrome.

An in-frame deletion in Kir6.2 (KCNJ11) causing neonatal diabetes reveals a site of interaction between Kir6.2 and SUR1 (2009)
Journal Article

Context: Activating mutations in genes encoding the Kir6.2 (KCNJ11) and SUR1 (ABCC8) subunits of the pancreatic ATP-sensitive K(+) channel are a common cause of permanent neonatal diabetes (PNDM). All Kir6.2 mutations identified to date are missense... Read More about An in-frame deletion in Kir6.2 (KCNJ11) causing neonatal diabetes reveals a site of interaction between Kir6.2 and SUR1.

SUR1: A unique ATP-binding cassette protein that functions as an ion channel regulator (2008)
Journal Article

SUR1 is an ATP-binding cassette (ABC) transporter with a novel function. In contrast to other ABC proteins, it serves as the regulatory subunit of an ion channel. The ATP-sensitive (KATP) channel is an octameric complex of four pore-forming Kir6.2 su... Read More about SUR1: A unique ATP-binding cassette protein that functions as an ion channel regulator.

How ATP inhibits the open KATP channel (2008)
Journal Article

ATP-sensitive potassium (KATP) channels are composed of four pore-forming Kir6.2 subunits and four regulatory SUR1 subunits. Binding of ATP to Kir6.2 leads to inhibition of channel activity. Because there are four subunits and thus four ATP-binding s... Read More about How ATP inhibits the open KATP channel.

Variable phenotypic spectrum of diabetes mellitus in a family carrying a novel KCNJ11 gene mutation (2008)
Journal Article

Aims: Heterozygous activating mutations in KCNJ11, which encodes the Kir6.2 subunit of the pancreatic ATP-sensitive potassium (K(ATP)) channel, cause both permanent and transient neonatal diabetes. Identification of KCNJ11 mutations has important the... Read More about Variable phenotypic spectrum of diabetes mellitus in a family carrying a novel KCNJ11 gene mutation.

Identification of the PIP2-binding site on Kir6.2 by molecular modelling and functional analysis (2007)
Journal Article

ATP-sensitive potassium (K(ATP)) channels couple cell metabolism to electrical activity by regulating K(+) fluxes across the plasma membrane. Channel closure is facilitated by ATP, which binds to the pore-forming subunit (Kir6.2). Conversely, channel... Read More about Identification of the PIP2-binding site on Kir6.2 by molecular modelling and functional analysis.

Mechanism of action of a sulphonylurea receptor SUR1 mutation (F132L) that causes DEND syndrome (2007)
Journal Article

Activating mutations in the genes encoding the ATP-sensitive potassium (K(ATP)) channel subunits Kir6.2 and SUR1 are a common cause of neonatal diabetes. Here, we analyse the molecular mechanism of action of the heterozygous mutation F132L, which lie... Read More about Mechanism of action of a sulphonylurea receptor SUR1 mutation (F132L) that causes DEND syndrome.

Studies of the ATPase activity of the ABC protein SUR1 (2007)
Journal Article

The ATP-sensitive potassium (KATP) channel couples glucose metabolism to insulin secretion in pancreatic β-cells. It comprises regulatory sulfonylurea receptor 1 and pore-forming Kir6.2 subunits. Binding and/or hydrolysis of Mg-nucleotides at the nuc... Read More about Studies of the ATPase activity of the ABC protein SUR1.

Phosphomimetic mutation of Ser-187 of SNAP-25 increases both syntaxin binding and highly Ca2+-sensitive exocytosis (2007)
Journal Article

The phosphorylation targets that mediate the enhancement of exocytosis by PKC are unknown. PKC phosporylates the SNARE protein SNAP-25 at Ser-187. We expressed mutants of SNAP-25 using the Semliki Forest Virus system in bovine adrenal chromaffin cell... Read More about Phosphomimetic mutation of Ser-187 of SNAP-25 increases both syntaxin binding and highly Ca2+-sensitive exocytosis.